A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Berberine Chloride and Guggulsterone Z in Herbal Formulation

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Published: May 10, 2019

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Authors

Vijaykumar K. Parmar Department of Pharmaceutical Sciences, Sardar Patel University, VallabhVidyanagar – 388 120, Gujarat, INDIA
Deepika Mohanta Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa – 388421, Gujarat, INDIA
Harsh Shah Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Changa – 388421, Gujarat, INDIA

DOI Number
https://doi.org/https://doi.org/10.15415/jptrm.2019.71002

Keywords
Berberine chloride, Guggulsterone Z, HPTLC, Herbal formulation

Abstract

A simple, precise, and robust high-performance thin layer chromatography (HPTLC) method was developed and validated for the determination of berberine chloride and guggulsterone Z in herbal formulation. Chromatographic separation was achieved on aluminium plates precoated with silica gel G60F254 as the stationary phase and toluene-acetonitrile-formic acid (5:3:0.5 v/v/v) as the mobile phase. Densitometric evaluation was carried out at 264 nm. The present method was validated according to ICH guidelines. The Rf value of berberine chloride and guggulsterone Z was found to be 0.40 ± 0.02 and 0.68 ± 0.02, respectively. The response in terms of peak area was found to be linear over the concentration range of 100-500 ng/spot for berberine chloride and 200-1000 ng/spot for guggulsterone Z with regression coefficient value greater than 0.995 for both the phytoconstituents. The method was validated by determining its accuracy, precision, robustness, specificity and system suitability. The method was found to be accurate, precise and robust to carry out the simultaneous estimation of berberine chloride and guggulsterone Z. The developed method was successfully applied for the simultaneous estimation of berberine chloride and guggulsterone Z in herbal formulation.

References

ICH Q2(R1) guidelines, http://www.ich.org/products/guidelines/quality/article/quality-guidelines.html.
Variya K, Parmar V. K. (2015). A Validated High-Performance Thin-Layer Chromatographic Method for the quantitation of protodioscin in Trigonella foenum-graecum and its herbal formulation. Journal of Planar Chromatography. 6, 458–465. https://doi.org/10.1556/1006.2015.28.6.7
Patel H. N., Parmar V. K., Patel B. (2014). Sensitive and Robust Methods for Simultaneous Determination of Beclomethasone Dipropionate and Formoterol Fumarate Dihydrate in Rotacaps. Journal of Chromatographic Science. 1–12.https://doi.org/10.1093/chromsci/bmt208
Tarte P.S., Shedharkar G.R. (2014). Force Degradation Study of Berberine Chloride by Using Stability Indicating HPLC Method. International Journal of PharmTech Research. 6(5), 1490–1500.

How to Cite

Vijaykumar K. Parmar, Deepika Mohanta, Harsh Shah . A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Berberine Chloride and Guggulsterone Z in Herbal Formulation. J. Pharm. Technol. Res. Manag.. 2019, 07, 07-13

A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Berberine Chloride and Guggulsterone Z in Herbal Formulation

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