Phytochemical Screening, Chromatographic Determination and Antioxidant Profiling of Hydroethanolic Extract of Green Coffee Bean Extract

Home > 2024, Vol. 12 No. 01 > Phytochemical Screening, Chromatographic Determination and Antioxidant Profiling of Hydroethanolic Extract of Green Coffee Bean Extract

Published: April 20, 2024

Download PDF

Download HTML

Authors

Mamta Saini, Saurabh Gupta, Sushma Devi, Thakur Gurjeet singh

DOI Number
https://doi.org/10.15415/jprtm.2024.121004

Keywords
Green coffee extract, Phytochemical screening, TLC, HPTLC, Mass spectroscopy, Antioxidant profile

Abstract

Background: Green coffee beans are rich in bioactive compounds, particularly chlorogenic acids, which are known for their health benefits. Phytochemical screening and chromatographic techniques help identify and quantify these compounds. This research explores its phytochemical profile and antioxidant efficacy, contributing to its therapeutic relevance.

Purpose: The current work was conducted to inspect the chromatographic and spectroscopic profile, phytochemical constituents, and antioxidant activity of the hydroethanolic extract of green coffee Arabica.

Methods: The hydroethanolic extract of green coffee beans was extracted from shade-dried beans of green coffee Arabica using the Soxhlet extraction method. The bioactive compounds were identified using HPTLC. An analysis of the antioxidant profile of hydroethanolic GCBE and standards was carried out with the help of 2,2-Diphenyl-1-picryl-hydrazyl (DPPH), 2,2′-casino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS), nitric oxide radical assay, superoxide radical test, and lipid peroxidation.

Results: The qualitative phytochemical analysis displayed that the hydroethanolic extract of green coffee beans consisted of carbohydrates, phenolic compounds, saponins, alkaloids, and flavonoids. The presence of chlorogenic acid and trigonelline (R_Fvalue 0.32 and 0.16) was confirmed by comparing it with standards (R_Fvalue 0.35 and 0.17), respectively. The total phenolic and flavonoid contents of GCBE were found to be 225.6 ± 0.37 & 42.53 ± 0.34 mg /g, respectively. The extract showed significant antioxidant activity.

Conclusion: The hydroethanolic extract of green coffee beans had significant antioxidant activity and confirmed the presence of trigonelline, caffeic acid, chlorogenic acid, and quinic acid. It was concluded that due to the presence of a substantial amount of phenolic compounds, it showed considerable antioxidant activity.

References

Belviso, S., & Barbosa-Pereira, L. (2019). Coffee Supplements. Nonvitamin and Nonmineral Nutritional Supplements, 177-185. Academic Press. https://doi.org/10.1016/B978-0-12-812491-8.00025-4
Bertrand, B., Boulanger, R., Dussert, S., Ribeyre, F., Berthiot, L., Descroix, F., & Joët, T. (2012). Food Chemistry, 135(4), 2575–2583. https://doi.org/10.1016/j.foodchem.2012.06.060
Caldarelli, C. E., Gilio, L., & Zilberman, D. (2019). The Coffee Market in Brazil: challenges and policy guidelines. Revista de Economia, 39(69). https://doi.org/10.1007/s00764-023-00230-7
Clarke, R. J. (2003). Coffee: Roast and ground. Encyclopedia of Food Science and Nutrition, 1487–1493. https://doi.org/10.1016/B0-12-227055-X/00269-8
Cordoba, N., Fernandez-Alduenda, M., Moreno, F. L., & Ruiz, Y. (2020). Coffee extraction: A review of parameters and their influence on the physicochemical characteristics and flavour of coffee brews. Trends in Food Science & Technology, 96, 45-60. https://doi.org/10.1016/j.tifs.2019.12.004
Damatta, F. M., & Ramalho, J. C. (2006). Impacts of drought and temperature stress on coffee physiology and production: A review. Brazilian Journal of Plant Physiology, 18, 55–81.
Dos Santos, É. M., de Macedo, L. M., Ataide, J. A., Delafiori, J., de Oliveira Guarnieri, J. P., Rosa, P. C. P., & Mazzola, P. G. (2024). Antioxidant, antimicrobial and healing properties of an extract from coffee pulp for the development of a phytocosmetic. Scientific reports, 14(1), 4453. https://doi.org/10.1038/s41598-024-54797-0
Fischer, M., Reimann, S., Trovato, V., & Redgwell, R. J. (2011). Polysaccharides of green arabica and robusta coffee beans. Carbohydrate Research, 330, 93–101.
Garg, S. K., Shukla, A., & Choudhury, S. (2021). Green coffee beans. Nutraceuticals, 725-748. Academic Press. https://doi.org/10.1016/B978-0-12-821038-3.00042-2
Gupta, S., Saini, M., & Singh, T. G. (2020). Updated pharmacological, clinical, and phytochemical prospects of green coffee: A review. Plant Archives, 20, 3820–3827.
International Coffee Organization. (2011). Breakdown of exports of green Arabica and green Robusta of countries exporting significant volumes of both types of coffee. Statistics. http://www.ico.org
Kasahun, W., Kassaye, T., Minaleshewa, A., Bewketu, M., Melkamu, A., Daniel, A. M., & Bizuayehu, K. (2024). Level of caffeine, trigonelline, and chlorogenic acids in green coffee (Coffea arabica L.) beans from Amhara region, Ethiopia. Journal of Agricultural and Food Research, 16, 101082. https://doi.org/10.1016/j.jafr.2024.101082
Lalhminghlui, K., & Jagetia, G. C. (2018). Evaluation of the free-radical scavenging and antioxidant activities of Chilauni, Schima wallichii Korth in vitro. Future Science OA, 4(2), FSO272. https://doi.org/10.4155/fsoa-2017-0086
Lombo, V. O., Freitas, S., Bizzo, H., Ferreira, M., & Rezende, C. (2022). Sustainable utilization of cold-pressed green coffee oil and its by-products for high-value materials. Journal of Food Processing and Preservation, 46(8). https://doi.org/10.1111/jfpp.16361
Madaan, R., Bansal, G., Kumar, S., & Sharma, A. (2011). Estimation of total phenols and flavonoids in extracts of Actaea spicata roots and antioxidant activity studies. Indian Journal of Pharmaceutical Sciences, 73(6), 666–669. https://doi.org/10.4103/0250-474X.100242
Marcocci, L., Packer, L., Droy-Lefaix, M. T., Sekaki, A., & Gardes-Albert, M. (1994). Antioxidant action of Ginkgo biloba extracts EGB 761. Methods in Enzymology, 234, 462–475.
Masek, A., Latos-Brozio, M., Kałużna-Czaplińska, J., Rosiak, A., & Chrzescijanska, E. (2020). Antioxidant properties of green coffee extract. Forests, 11(5), 557.
Masood, S., Shafi, N., Amin, S., Arshad, H., Ahmed, N., Khattak, R., Fareed, K., & Yar, R. (2021). Analytical techniques and herbal medicinal plants: A review of past and present techniques. Advances in Environmental Science and Technology, 1(2), 90. https://doi.org/10.15228/2021.v01.i02.p90-102
Moreira, M. E. D. C. (2013). Evaluation of the pharmacological potential of green and roasted coffee (Coffea arabica L.). http://www.sbicafe.ufv.br/handle/123456789/6612
Munteanu, I. G., & Apetrei, C. (2021). Analytical methods used in determining antioxidant activity: A review. International Journal of Molecular Sciences, 22(7), 3380. https://doi.org/10.3390/ijms22073380
Nortjie, E., Basitere, M., Moyo, D., & Nyamukamba, P. (2022). Extraction methods, quantitative and qualitative phytochemical screening of medicinal plants for antimicrobial textiles: A review. Plants (Basel, Switzerland, 11(15), 2011. https://doi.org/10.3390/plants11152011
Onakpoya, I., Terry, R., & Ernst, E. (2011). The use of green coffee extract as a weight loss supplement: A systematic review and meta-analysis of randomized clinical trials. Gastroenterology Research and Practice, 2011, 382852. https://doi.org/10.1155/2011/382852
Pagare, S., Bhatia, M., Tripathi, N., Pagare, S., & Bansal, Y. K. (2015). Secondary metabolites of plants and their role: Overview. Current trends in biotechnology and pharmacy, 9(3), 293-304.
Pisoschi, A. M., Pop, A., Cimpeanu, C., & Predoi, G. (2016). Antioxidant capacity determination in plants and plant-derived products: A review. Oxidative Medicine and Cellular Longevity, 2016, 9130976. https://doi.org/10.1155/2016/9130976
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237.
Saud, S., & Salamatullah, A. M. (2021). Relationship between the chemical composition and the biological functions of coffee. Molecules, 26, 7634. https://doi.org/10.3390/molecules26247634
Siddiqui, M. R., Al Othman, Z. A., & Rahman, N. (2017). Analytical techniques in pharmaceutical analysis: A review. Arabian Journal of Chemistry, 10, S1409–S1421.
Toledo, P. R., Pezza, L., Pezza, H. R., & Toci, A. T. (2016). Relationship between the different aspects related to coffee quality and their volatile compounds. Comprehensive Reviews in Food Science and Food Safety, 15(4), 705-719. https://doi.org/10.1111/1541-4337.12205

How to Cite

Mamta Saini, Saurabh Gupta, Sushma Devi, Thakur Gurjeet singh . Phytochemical Screening, Chromatographic Determination and Antioxidant Profiling of Hydroethanolic Extract of Green Coffee Bean Extract. J. Pharm. Technol. Res. Manag.. 2024, 12, 52-61

Phytochemical Screening, Chromatographic Determination and Antioxidant Profiling of Hydroethanolic Extract of Green Coffee Bean Extract

Current Issue

Periodicity	Biannually
Issue-1	May
Issue-2	November
ISSN Print	2321-2217
ISSN Online	2321-2225
RNI No.	CHAENG/2013/50088

OA Policy

Publisher's policy of the journal at Sherpa UK for the submitted, accepted, and published articles. Click OAPolicy

Plan-S Compliance

To check compliance, one has to use the Journal Check Tool (JCT). This tool provided by cOAlition S (European funders) for the researchers (fundee) to check the compliance with the journal.

Recommend journal to your library

You can recommend the journal being a researcher or faculty member to your library. We will post a copy of the Journal to your library on your behalf at free of cost.
Click here: Recommend Journal

Preprint Arxiv Submission

The authors are encouraged to submit the author’s copy (preprint) to appropriate preprint archives e.g. https://arxiv.org and/or on https://indiarxiv.org or institutional repositories (e.g., D Space) before paper acceptance by the editor of Journal. After publications of the paper author(s) should mention the citation information, title and abstract along with DOI number of the publication carefully on the required page of the depository(ies).

Contact: Phone: +91-172-2741000, +91-172-4691800

Email : editor.jptrm@chitkara.edu.in;

Abstract and Indexing

Information

This work is licensed under a Creative Commons Attribution 4.0 International License.

Articles in Journal of Pharmaceutical Technology, Research and Management (J. Pharm. Tech. Res. Management) by Chitkara University Publications are Open Access articles that are published with licensed under a Creative Commons Attribution- CC-BY 4.0 International License. Based on a work at https://jptrm.chitkara.edu.in/. This license permits one to use, remix, tweak and reproduction in any medium, even commercially provided one give credit for the original creation.

View Legal Code of the above-mentioned license, https://creativecommons.org/licenses/by/4.0/legalcode

View Licence Deed here https://creativecommons.org/licenses/by/4.0/