Phytochemical Screening, Chromatographic Determination and Antioxidant Profiling of Hydroethanolic Extract of Green Coffee Bean Extract

Abstract

Background: Green coffee beans are rich in bioactive compounds, particularly chlorogenic acids, which are known for their health benefits. Phytochemical screening and chromatographic techniques help identify and quantify these compounds. This research explores its phytochemical profile and antioxidant efficacy, contributing to its therapeutic relevance.

Purpose: The current work was conducted to inspect the chromatographic and spectroscopic profile, phytochemical constituents, and antioxidant activity of the hydroethanolic extract of green coffee Arabica.

Methods: The hydroethanolic extract of green coffee beans was extracted from shade-dried beans of green coffee Arabica using the Soxhlet extraction method. The bioactive compounds were identified using HPTLC. An analysis of the antioxidant profile of hydroethanolic GCBE and standards was carried out with the help of 2,2-Diphenyl-1-picryl-hydrazyl (DPPH), 2,2'-casino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS), nitric oxide radical assay, superoxide radical test, and lipid peroxidation.

Results: The qualitative phytochemical analysis displayed that the hydroethanolic extract of green coffee beans consisted of carbohydrates, phenolic compounds, saponins, alkaloids, and flavonoids. The presence of chlorogenic acid and trigonelline (RF value 0.32 and 0.16) was confirmed by comparing it with standards (RF value 0.35 and 0.17), respectively. The total phenolic and flavonoid contents of GCBE were found to be 225.6 ± 0.37 & 42.53 ± 0.34 mg /g, respectively. The extract showed significant antioxidant activity.

Conclusion: The hydroethanolic extract of green coffee beans had significant antioxidant activity and confirmed the presence of trigonelline, caffeic acid, chlorogenic acid, and quinic acid. It was concluded that due to the presence of a substantial amount of phenolic compounds, it showed considerable antioxidant activity.

  • Page Number : 52-61
  • Published Date : 2024-04-20
  • Keywords
    Green coffee extract, Phytochemical screening, TLC, HPTLC, Mass spectroscopy, Antioxidant profile
  • DOI Number
    10.15415/jprtm.2024.121004
  • Authors
    Mamta Saini, Saurabh Gupta, Sushma Devi, Thakur Gurjeet singh

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