A Coherent Analytical Review of Indapamide

Published: May 7, 2022


  • A.P. Rajput
  • A.P. Edlabadkar
Indapamide; Diuretic; PharmaceuticalAnalytical Profile; Review


Indapamide is a diuretic of particular importance in an antihypertensive therapeutic regime that is profoundly applied to treat hypertension. Besides using as an individual drug for its diuretic and thereby antihypertensive effect, the drug is also a part of crucial combinations with Angiotensin-Converting Enzyme inhibitors. The recent combinations of Indapamide with Perindopril and Delapril work as the best therapies for heart patients.
Considering the therapeutic and pharmaceutical importance of the drug molecule and its potential to be explored in future drug discovery and delivery, we made efforts to portray the pharmaceutical analytical profile of the drug molecule at ease.
The present article is a composition entailing the comparative and critical evaluation of the various analytical approaches explored for the pharmaceutical estimation ofIndapamide as a particular drug in combinations and pharmaceutical as well as biological matrices. The analyses are reviewed for the specificapplications of analytical methods tested Indapamide alone or with other medications.
The anticipated study revealed the comparative usage of different research techniques for Indapamide estimation. The study influences the possibility of constraints and focuses on the recent trend in Indapamide analysis. The article also provides an understanding of sample processing, drug extraction method, validation protocols/approaches, and stability studies, including but not limited to the bioanalytical perspectives.
This review can be explored thoroughly for the future analytical and pharmaceutical insights of Indapamide to the fullest.


Al-Tannak, N. F. (2018). UHPLC-UV method for simultaneous determination of perindopril arginine and indapamide hemihydrate in combined dosage form: a stability-indicating assay method. Scientia Pharmaceutica, 86(1), 7. https://doi.org/10.3390/scipharm86010007

Asmar, R. G., London, G. M., O’Rourke, M. E., Safar, M. E., & REASON Project Coordinators and Investigators. (2001). Improvement in blood pressure, arterial stiffness and wave reflections with a very-low-dose perindopril/indapamide combination in hypertensive patient: a comparison with atenolol. Hypertension, 38(4), 922-926.

Basu, A., Das, B., Basak, K., Chakraborty, K., &Basu, S. (2011). Development & validation of stability indicating high performance liquid chromatographic method for simultaneous estimation of atenolol & indapamide in tablet dosage form. Journal of Pharmacy Research, 4(6), 1677-1680.

Bataillard, A., Schiavi, P., &Sassard, J. (1999). Pharmacological properties of indapamide. Clinical pharmacokinetics, 37(1), 7-12.

Bhoir, V.; Hibare, V.; &Damle, M. C. (2014) Development and validation of stability-indicating HPTLC method for the estimation of perindopril and Indapamide. International Journal of Pharmacy and Pharmaceutical Sciences.6, 621-5.

British Pharmacopoeia, Vol I. The British Pharmacopoeia Commission, London; 2022. p. 1313 – 1314.

Budavari, S., O’Neil, M. J., Smith, A., &Heckelman, P. E. (1996). The merck index 12th Ed(pp. 1103, 6513). Rahway, NJ: Merck.

Cârje, A. G., Ion, V., Muntean, D. L., Hancu, G., Balint, A. L. I. N. A., &Imre, S. I. L. V. I. A. (2016). Enantioseparation of indapamide by high performance liquid chromatography using ovomucoid glycoprotein as chiral selector. Farmacia64, 181-186.

Cavalieri, L., &Cremonesi, G. (2007). Delapril plus Indapamide. Clinical drug investigation, 27(6), 367-380.

Chavda, J. M.; Raval, M. A.; & Dave, K. R. (2012) Simultaneous estimation of Telmisartan and Indapamide in Bulk Drug and in pharmaceutical dosage form using UV-Spectrophotometry.Pharm Methods.5, 4169.

Chen, W.; Liang, Y.; Zhang, H.; Li, H.; Xiong, Y.; Wang, G. &Xie, L. (2006) Simple, sensitive and rapid LC – MS method for the quantitation of indapamide in human plasma — application to pharmacokinetic studies.Journal of Chromatography B.842, 58–63. https://doi.org/10.1016/j.jchromb.2006.03.024

Dawud, E. R., & Shakya, A. K. (2019). HPLC-PDA analysis of ACE-inhibitors, hydrochlorothiazide and indapamide utilizing design of experiments. Arabian Journal of chemistry, 12(5), 718-728.https://doi.org/10.1016/j.arabjc.2014.10.052

Desai, A. K.; Chauhan, R. S.; Shah, S. A.; Shah, D. R. (2012) HPTLC Method for the Simultaneous Estimation of Amlodipine Besylate and Indapamide in Tablet Formulation.Asian Journal of Research in Chemistry.5, 510-514.

Ding, L.; Yang, L.; Liu, F.; Ju, W.; &Xiong, N. (2006) A sensitive LC – ESI-MS method for the determination of indapamide in human plasma : Method and clinical applications.Journal of pharmaceutical and Biomedical analysis. 42, 213–217. https://doi.org/10.1016/j.jpba.2006.03.039

Divitiis, O. D., Somma, S. D., Petitto, M., Fazio, S., &Ligouri, V. (1983). Indapamide and atenolol in the treatment of hypertension: double-blind comparative and combination study. Current Medical Research and Opinion, 8(7), 493-500.

El-Bagary, R. I., Elkady, E. F., Mowaka, S., &Attallah, M. A. (2017). A validated HPLC method for simultaneous determination of perindopril arginine, amlodipine, and indapamide: application in bulk and in different pharmaceutical dosage forms. Journal of AOAC International, 100(4), 992-999.

El-Gindy, A., Nassar, M. W., Attia, K. A. S., Abu-Seada, H. H., & El-Ghandour, M. (2014). Stability-Indicating HPLC method for simultaneous determination of captopril, indapamide, and their related compounds. Journal of Liquid Chromatography & Related Technologies37(5), 696-712.https://doi.org/10.1080/10826076.2012.758138.

Erk, N. (2001). Comparison of spectrophotometric and an LC method for the determination perindopril and indapamide in pharmaceutical formulations. Journal of pharmaceutical and Biomedical Analysis26(1), 43-52.

Ernst, M. E., &Fravel, M. A. (2022). Thiazide and the Thiazide-Like Diuretics: Review of Hydrochlorothiazide, Chlorthalidone, and Indapamide. American Journal of Hypertension.

Ezzeldin, M. I., Shokry, E., Fouad, M. A., &Elbagary, R. I. (2013). Application of chromatographic and spectrophotometric methods for the analysis of aliskiren and hydrochlorothiazide antihypertensive combination. International journal of advanced chemistry, 1(2), 13-20.

Fernandes, N., Nimdeo, M. S., Choudhari, V. P., Kulkarni, R. R., Pande, V. V., &Nikalje, A. G. (2008). Dual wavelength and simultaneous equation spectrophotometric methods for estimation of atenolol and indapamide in their combined dosage form. Int J Chem Sci6(1), 29-35.

Fernandez-Carballido, A., Barcia, E., Cordoba-Diaz, D., Cordoba-Diaz, M., & Negro, S. (2014). Lisinopril-loaded chitosan nanoparticles and indapamide in hard gelatine capsules: simultaneous HPLC quantification. Current pharmaceutical Analysis10(1), 10-19.

Gao, X.; Chen, J.; Mei, N.; Tao, W.; Jiang, W.; & Jiang, X. (2005) HPLC Determination and Pharmacokinetic Study of Indapamide in Human Whole Blood.Chromatographia. 581–582. https://doi.org/10.1365/s10337-005-0548-1

Gumieniczek, A., Mączka, P., Komsta, Ł., &Pietraś, R. (2015). Dissolution profiles of perindopril and indapamide in their fixed-dose formulations by a new HPLC method and different mathematical approaches. Acta Pharmaceutica65(3), 235-252.https://doi.org/10.1515/acph-2015-0024

Guo, Y.; Ni, Y.; Chen, J.; Kokot, S. A (2016) Kinetic spectrofluorometric method, aided by chemometrics, for the analysis of Sibutramine, Indapamide and hydrochlorothiazide compounds found in weight-reducing tonic samples. Analytical Methods. 8, 197-204.

Hegheș, S. C., Rus, L. M., Rus, L. L., Bojiță, M. T., &Iuga, C. A. (2017). HPLC-UV determination of indapamide in the presence of its main synthesis and degradation impurities. Method validation. Farmacia65(5), 755-760.

Indian Pharmacopoeia. Vol. II. The Indian pharmacopoeia commission. Central Indian Pharmacopoeia Laboratory, Ministry of Health and Family Welfare, Govt of India, Sector. 2018, p. 2283-2287

Jadhav, U., Hiremath, J., Namjoshi, D. J., Gujral, V. K., Tripathi, K. K., Siraj, M., … & Safar, M. (2014). Blood pressure control with a single-pill combination of indapamide sustained-release and amlodipine in patients with hypertension: the EFFICIENT study. PLos one, 9(4), e92955.

Jain, D. S.; Subbaiah, G.; Sanyal, M.; Pande, U. C.; &Shrivastav, P. (2006) Liquid chromatography – tandem mass spectrometry validated method for the estimation of indapamide in human whole blood.Journal of Chromatography B.834, 149–154. https://doi.org/10.1016/j.jchromb.2006.02.040

Kadian, N., Maste, M., Bhat, A. R., Pujari, P., Ramesh, V., & Bhatt, A. R. (2012). An Effective RP-HPLC Method for the Simultaneous Determination of Atenolol and Indapamide in Marketed Tablet Formulation (ATEN-D). ANALYST1(7.57), 8-98.

Karunakar, N.; Puranik, S. B.; Kumar, G.V.S.; Masood, M.D.; Sridhar, K.A. (2012) Development and Validation of RP-HPLC Method for Simultaneous Estimation of Amlodipine and Indapamide in Bulk and Tablet Dosage Form. Asian Journal of Biomedical and Pharmaceutical Sciences.2(12), 55-59.

Madhavi, K.; Deepti, K.; &Harika, B. (2014). RP-HPLC Method Development and Validation for the Simultaneous Estimation of Atenolol and Indapamide in Pharmaceutical Tablet Dosage Form. International Journal of Pharmacy and Analytical Research, 3, 109–117.

Mahant, B. D., Rajoriya, V., Jain, R. K., &Kashaw, V. (2016). UV-Spectrophotometric and Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Amlodipine Besylate and Indapamide. Analytical Chemistry Letters6(4), 354-370.https://doi.org/10.1080/22297928.2016.1217787

Miller, R. B., Dadgar, D., &Lalande, M. (1993). High-performance liquid chromatographic method for the determination of indapamide in human whole blood. Journal of Chromatography B: Biomedical Sciences and Applications614(2), 293-298.

Modi, D. K.; & Patel, C. N. (2011) Development and Validation of Spectrophotometric Method for Simultaneous Estimation of Perindopril and Indapamide in Combined. Journal of AOAC International.6, 46–52.

Morihisa, H.; Fukata, F.; Muro, H.; Nishimura, K.; & Makino, T. (2008) Determination of indapamide in human serum using 96-well solid-phase extraction and high-performance liquid chromatography – tandem mass spectrometry (LC – MS / MS). Journal of chromatography B.870, 126–130. https://doi.org/10.1016/j.jchromb.2008.05.042

Murray, G. J.; &Danaceau, J. P. (2009) Simultaneous extraction and screening of diuretics, beta-blockers, selected stimulants and steroids in human urine by HPLC-MS / MS and UPLC-MS / MS. Journal of Chromatography B. 877, 3857–3864. https://doi.org/10.1016/j.jchromb.2009.09.036

Nakov, N.; Mladenovska, K.; Labacevski, N.; Dimovski, A.; Petkovska, R., Dimitrovska, A., &Kavrakovski, Z. (2013) Development and validation of automated SPE-LC-MS / MS method for determination of indapamide in human whole blood and its application to real study samples.Biomedical Chromatography. 27,1540–1546. https://doi.org/10.1002/bmc.2957

Nakov, N.; Mladenovska, K.; Zafirov, D.; Dimovski, A.; Petkovska, R.; Dimitrovska, A.; &Kavrakovski, Z. (2013) High-throughput SPE-LC-MS / MS method for determination of indapamide in human serum. Macedonian Pharmaceutical Association. 59, 15–22.

Nazareth, C.; Shivakumar, B.; Reddy, P.; Gurupadayya, B. M. (2013) Development and validation of HPTLC method for simultaneous analysis of olmesartan and indapamide in bulk drug and combined tablet formulation, Indo American Journal of Pharmaceutical Research.3,(12) 1709-1715.

Ozden, T., TURKER, Z. H., &Tosun, A. U. (1998). Quantitative High‐performance Liquid Chromatographic Analysis of Indapamide in Pharmaceutical Dosage Forms. Pharmacy and Pharmacology Communications, 4(8), 397-399.

Pai, J. B., Shetty, S., Chenna, G., Gopinath, B., & Ahmed, M. (2011). Development of new spectrophotometric methods for the determination of Indapamide in bulk and pharmaceutical formulations. International Journal of ChemTech Research3(2), 755-760.

Pai, J. B.; Shetty, A. S. K.; &Gopinath, B. (2011) Development and Validation of RP-HPLC Method for Quantitative estimation of Indapamide in Bulk and Pharmaceutical dosage forms. International Journal of pharmtech Research.3, 1482–1487.

Parusu, T., &Ponneri, V. (2012). RP-HPLC method for simultaneous determination of atenolol and indapamide in pharmaceutical dosage forms, human blood and milk. European Journal of chemistry3(2), 138-142.https://doi.org/10.5155/eurjchem.3.2.138-142.537

Patel, D. B., Mehta, F. A., & Bhatt, K. K. (2012). Simultaneous estimation of amlodipine besylate and indapamide in a pharmaceutical formulation by a high performance liquid chromatographic (RP-HPLC) method. Scientia Pharmaceutica80(3), 581-590.

Patel, N. M.; Raval, M. A.; Shah, K. A. (2013) Development of Stability Indicating HPTLC Method For Simultaneous Estimation Of Telmisartan And Indapamide In Bulk Drug And Pharmaceutical Dosage Form, International Journal of PharmtechReserach, 5(4), 1546–1553.

Patel, P. K.; Parmar, R. R.; Shah, V. N.; & Shah, D. A. (2012).Simultaneous estimation of telmisartan and indapamide in pharmaceutical dosage form by UV spectrophotometric method.International Journal of Institutional Pharmacy and Life Sciences,2, 50–57.

Pathak, A. K., Lodhi, R., &Shikhri, M. K. (2011). A selective reverse phase HPLC method for simultaneous determination of perindopril and indapamide in solid dosage form and bulk drug. J. Pharm. Res4(8), 2512-2513.

Pinto, G.A.; Pastre, K.I.; Bellorio, K.B.; de Souza Teixeira L, de Souza WC, de Abreu FC, de Santana e Silva Cardoso FF, Pianetti GA, César IC. (2014) An improved LC‐MS/MS method for quantitation of indapamide in whole blood: application for a bioequivalence study. Biomedical Chromatography. 28 (9), 1212-8.

Raj, M. C.; Chaudhari, B.G. (2012)Development and Validation of RP-HPLC Method for Simultaneous Estimation of Amlodipine Besylate and Indapamide in Tablet Dosage Form. International Journal of Pharmaceutical Science and Research. 3(9), 3146-3150

Rani, G. T., Sankar, D. G., Kadgapathi, P., & Satyanarayana, B. (2011). A validated RP-HPLC method for simultaneous estimation of atenolol and indapamide in pharmaceutical formulations. E-Journal of chemistry8(3), 1238-1245.

Rao, J. R. (2011) Simultaneous HPTLC analysis of atenolol and indapamide in tablet formulation Int J Compr Pharm.9, 1-4.

Saleh HM, Amin AS, El-Mammli M. (2001) New colorimetric methods for the determination of indapamide and its formulations. Microchimica Acta. 1;137(3-4):185-9.

Saujanya S.; (2017) Method Development and Validation of Simultaneous Estimation of Perindopril and Indapamide in Tablet By RP-HPLC Method, Indo American Journal of Pharmaceutical Research. 7(9), 390-397

Shah, R. N., Gandhi, D. B., & Patel, M. M. (2012). RP-HPLC method for simultaneous estimation of Amlodipine Besylate and Indapamide in tablet dosage form. Asian Journal of Research in Chemistry5(5), 8.

Shah, R. N., Gandhi, D. B., & Patel, M. M. (2012). Simultaneous determination of Amlodipine Besylate and Indapamide in tablet dosage form by Absorption correction method and First order derivative UV spectroscopy. Int J PharmTech Res4(3), 1018-24.

Sheth, A., Patel, K. N., Badmanaban, R., Doshi, N., & Shah, N. (2013). Simultaneous estimation of olmesartanmedoxomil and indapamide from bulk and commercial products using a validated reverse phase high performance liquid chromatographic technique. Chronicles of young scientists4(1).

Singhvi, I.; Goyal, A. (2007) Visible Spectrophotometric Estimation of Aceclofenac and Indapamide from Tablets using Tablets Folin-Ciocalteu Reagent. Indian Journal of Pharmaceutical Sciences. 69(1), 164-165.

Sîrbu, C.; Tomuta, I.; Achim, M.; Rus, L.; Vonica, L.; & Dinte, E. (2014) Quantitative characterization of powder blends for tablets with indapamide by near-infrared spectroscopy and chemometry.Farmacia. 62, 48–57.

Suo, X., Deng, Y., & Hao, A. (2005). Determination of lauroyl-indapamide in rat whole blood by high-performance liquid chromatography. Journal of Chromatography B819(1), 191-196..https://doi.org/10.1016/j.jchromb.2005.01.032

Süslü, I.; Altınöz, S. (2002) Two derivative spectrophotometric determinations of indapamide in pharmaceutical dosage forms.Journal of pharmaceutical and biomedical analysis,30(2), 357–364.

Tang, J.; Li, J.; Sun, J.; Yin, J.; & He, Z. (2005) Rapid and sensitive determination of indapamide in human blood by liquid chromatography with electrospray ionization mass spectrometric detec- tion : application to a bioequivalence study,DiePharmazie-An International Journal of Pharmaceutical Sciences.60, 819–822.

Tarkase, K. N.; Jadhav M. B.; Tajane S. R.; Dongare U. S. (2012) Development and Validation of UV-Spectrophotometric methods for estimation of Indapamide in bulk and tablet dosage form. Der Pharma Chemica, 4 (3), 1128-1132.

The United State Pharmacopoeia, USP41-NF36, United State Pharmacopoeial Convention, Inc., Rockville MD 2018. p. 2139-40.

Valentin, I., Silvia, I., Gabriela, C. A., & Lucia, M. D. (2015). Analytical Performance of an HPLC and CZE Methods for the Analysis and Separation of Perindopril Erbumine and Indapamide. Acta Marisiensis-Seria Medica, 61(4), 324-327.https://doi.org/10.1515/amma-2015-0073

Vyas, N.; Panchal, S. (2012) A Validated HPTLC Method for Simultaneous Estimation of Nebivolol and Indapamide in Solid Dosage Form. Asian Journal of Pharmaceutical and Clinical Research. 5(4),215-2018.

Waeber, B. (2003). Combination therapy with ACE inhibitors/angiotensin II receptor antagonists and diuretics in hypertension. Expert Review of Cardiovascular Therapy, 1(1), 43-50.

Woo H, Kim JW, Han KM, Lee JH, Hwang IS, Lee JH, Kim J, Kweon SJ, Cho S, Chae KR, Han SY.(2013) Simultaneous analysis of 17 diuretics in dietary supplements by HPLC and LC-MS / MS.Food Additives & Contaminants: Part A.30 (2), 209-217.

Zeng, Y.; Xu, Y., Kee; C., Low, M.; &Ge, X. (2016) Analysis of 40 weight loss compounds adulterated in health supplements by liquid chromatography quadrupole linear ion trap mass spectrometry.Drug testing and analysis.8,351–356. https://doi.org/10.1002/dta.1846

Zhao L, Gu S, Xu R, Cui X, Gan F, Chen H. (2010) Comparison of liquid chromatography-ultraviolet and chromatography-tandem mass spectrometry for the determination of indapamide in human whole blood and their applications in bioequivalence studies. Drug Research. 60(07):432-9.

How to Cite

A.P. Rajput and A.P. Edlabadkar. A Coherent Analytical Review of Indapamide. J. Pharm. Technol. Res. Manag.. 2022, 10, 21-35
A Coherent Analytical Review of Indapamide

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