The plant Justicia neesii is rich in polyphenolic compounds like lignans which can exhibit anti-inflammatory properties. In this study ethanolic extracts of Justicia neesii was screened for elucidating its antioxidant potential using various methods. In all the methods it has shown good antioxidant nature and reducing ability. The IC50 values against DPPH, H2O2, NO and lipid peroxidases were found to be 55.72μg/mL, 133.35μg/mL, 29.65μg/mL and 271.02μg/mL respectively. The total phenolic content in the ethanolic extract was found to be 99.8mg per gram dry plant in gallic acid equivalents. The anti-inflammatory nature of Justicia neesii was tested using carrageenan induced paw edema model in rats. The extract has shown significant inhibition of paw volume at all the three gradient doses of the extract. The percent inhibition of paw volume is correlating with the standard diclofinac sodium. From this study we concluded that Justicia neesii is a plant with potent anti-inflammatory molecules.
A anjaneya, S.R., Lin, Z., Sundar Rao, K., Sang Chul, J., Narsimha, R., John, B., (2012). Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content. BMC Complementary and Alternative Medicine, 12, 173. http://dx.doi.org/10.1186/1472-6882-12-173
Brand Williams, W., Cuverlier, M.E., Berset, C., (1995). Use of free radical method to evaluate antioxidant activity. Lebensmittel- Wissenschaft and Technology, 28, 25-30. http://dx.doi.org/10.1016/S0023-6438(95)80008-5
C crunkhorn, P., Meacock, S.C., (1971). Mediators of the inflammation induced in the rat paw by carrageenin. British Journal of Pharmacology, 42, 392-402. http://dx.doi.org/10.1111/j.1476-5381.1971.tb07124.x
Day, S.H., Chiu, N.Y., Tsao, L.T., Wang, J.P., Lin, C.N., (2000). New lignan glycosides with potent anti-inflammatory effect isolated from Justicia ciliata. Journal of Natural Products, 63, 1560-62. http://dx.doi.org/10.1021/np000191j
Dodda, R., Gottumukkala, V.S., (1998). Justicia lignans- V. Three new beta-apolignans from Justicia neesii Ramamoorthy. Tetrahedron, 54, 13227-36. http://dx.doi.org/10.1016/S0040-4020(98)00803-5
Dodda, R., Mulabagal, V., Gottumukkala, V. S., (1999). Justicia lignans: Part 4- Two new arylnaphthalide lignans from Justicia neesii Ramamoorthy. Indian Journal of Chemistry, 38B, 713-17.
Dreosti, I.E., (2000). Antioxidant polyphenols in tea, cocoa and wine. Nutrition, 16, 692-694. http://dx.doi.org/10.1016/S0899-9007(00)00304-X
Filomena, C., Silvio, S., Mariangela, M., Federica, M., Giancarlo, A.S., Dimitar, U., (2008). In vivo anti-inflammatory and in vitro antioxidant activities of Mediterranean dietary plants. Journal of Ethnopharmacology, 116, 144–51. http://dx.doi.org/10.1016/j.jep.2007.11.015
G geone, M. C., Antonio F de, C.A., (2012). Chemical constituents and biological activities of species of Justicia – a review. Brazilian Journal of Pharmacognosy, 22, 220-38.
http://dx.doi.org/10.1590/s0102-695×2011005000196
G goldschmidt, S., Renn, K., (1922). Zweiwertiger Stickstoff Uber das α, α-Diphenyl-β-trinitrophenyl-hydrazyl. (IV. Mitteilung über Amin-Oxydation). Berichte der deutschen Chemischen Gesellschaft A/B, 55, 628–43. http://dx.doi.org/10.1002/cber.19220550308
G gottumukkala, V.S., Dodda, R., Jakka, K., Jorge, I.J., (2001). Justicia lignans: Part 7-Two new diphyllin glycosides from Justicia neesii Ramamoorthy. Indian Journal of Chemistry, 40B, 313-19. http://dx.doi.org/10.1002/chin.200131221
K kaviarasan, S., Naik, G.H., Gangabagirathi, R., Anuradha, C.V., Priyadarshini, K.I., (2007). In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds. Food Chemistry, 103, 31-37. http://dx.doi.org/10.1016/j.foodchem.2006.05.064
K kerr, M.E., Bender, C.M., Monti, E.J., (1991). An introduction to oxygen free radicals. Heart and Lung, 25, 200-9. http://dx.doi.org/10.1016/S0147-9563(96)80030-6
K kovuru, G., Jakka, K., Raju, V.K., Dodda, R., Gottumukkala, V.S., (2001). Justicia lignans: Part 9- Two new lignans from Justicia neesii Ramamoorthy (White flower variety). Indian Journal of Chemistry, 40B, 596-600.
L lata, H., Ahuja, G.K., (2003). Role of free radicals in health and disease. Indian journal of physiology and allied sciences, 57, 124.
Marcocci, L., Maguire, J.J., Droy-Lefai, M.T., Parker, L., (1994). Antioxidant action of Ginkgo biloba extract EG B761. Biochemical and Biophysical Research Communications, 201, 748-55.http://dx.doi.org/10.1006/bbrc.1994.1764
Navarro, E., Alonso, S.J., Trujillo, J., Jorge, E., Pérez, C., (2001). Pharmacological effects of elenoside, an arylnaphthalene lignan. Biological and Pharmaceutical Bulletin, 24, 254- 58. http://dx.doi.org/10.1248/bpb.24.254
Ohkawa, H., Nobuko, O., Kunio, Y., (1979). Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95, 351-58. http://dx.doi.org/10.1016/0003-2697(79)90738-3
Oyaizu, M., (1986). Studies on product of browning reaction prepared from glucosamine. Japanese Journal of Nutrition, 44, 307-15. http://dx.doi.org/10.5264/eiyogakuzashi.44.307
Pandey, Tripathi., (2010). Antioxidant activity of tuberosin isolated from Pueraria tuberose Linn. Journal of inflammation, 7, 47. http://dx.doi.org/10.1186/1476-9255-7-47
Pascual, G., Glass, C.K., (2006). Nuclear receptors verses inflammation. Trends in Endocrinology and Metabolism, 17, 101-5. http://dx.doi.org/10.1016/j.tem.2006.08.005
Pradyuti, D., Padhi, S.B., (2013). Algae in the Production of Novel Antioxidants. The Journal of Free Radicals and Antioxidants, 139, 199-203.
Ruch, R.J., Chug, S.U., Klaunig, J.E., (1984). Spin trapping of superoxide and hydroxyl radicals. Methods in Enzymology, 105, 198-209. http://dx.doi.org/10.1016/S0076-6879(84)05026-6
Shingu, M., (1982). Study on the mechanism of oxygen radical induced inflammation. Japan journal of inflammation, 2, 367-68.
Slinkard, K., Singleton, V.L., (1977). Total phenol analysis: Automation and compression with manual methods. American Journal of Enology and Viticulture, 28, 49.
Sridhar, N., Surya kiran, B.V.V.S., Sasidhar, D., Kanthal, L.K., (2013). Comparative anti-inflammatory and anti-oxidant evaluation of Jotropha gossypifolia and Croton bonplandianum. International Journal of Research in Pharmacy and Science, 4, 16-27.
Venkatachalam, C., Jurjen, T., Florian, W., Xin, L., Sergey, S., Julian, T., (2013). Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics. Lab on a Chip, 13, 4740-44. http://dx.doi.org/10.1039/c3lc50945a
Vinegar, R., Schreiber, W., Hugo, R., (1969). Biphasic development of carrageenan edema in rats. Journal of Pharmacology and Experimental Therapeutics, 166, 96-103.
Wang, H., Liu, F., Yang, L., Zu, Y.G., Wang, H., Qu, S.Z., (2011). Oxidative stability of fish oil supplemented with carnosic acid compared with synthetic antioxidants during long-term storage. Food Chemistry, 128, 93-99. http://dx.doi.org/10.1016/j.foodchem.2011.02.082
Winter, C.A., Risley, E.A., Nuss, G.W., (1962). Carrageenin induced edema in hind paws of the rat as an assay for anti-inflammatory drugs. Proceedings of the Society for Experimental Biology and Medicine, 111, 544-47. http://dx.doi.org/10.3181/00379727-111-27849
Zhang, L., Ravipati, A.S., Koyyalamudi, S.R., Jeong, S.C., Reddy, N., Smith, P.T., (2011). Antioxidant and anti inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds. Journal of Agricultural and Food Chemistry, 59, 12361–67. http://dx.doi.org/10.1021/jf203146e
| Periodicity | Biannually |
| Issue-1 | May |
| Issue-2 | November |
| ISSN Print | 2321-2217 |
| ISSN Online | 2321-2225 |
| RNI No. | CHAENG/2013/50088 |
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