Analytical Method Development and Validation for Assay of Rufinamide Drug
Authors
- Jitender SinghLord Shiva College of Pharmacy, Sirsa, Haryana, India
- Sonia SangwanChitkara College of Pharmacy, Chitkara University, Rajpura, Punjab-140401
- Parul GroverLord Shiva College of Pharmacy, Sirsa, Haryana, India
- Lovekesh MehtaLord Shiva College of Pharmacy, Sirsa, Haryana, India
- Deepika KiranLord Shiva College of Pharmacy, Sirsa, Haryana, India
- Anju GoyalChitkara College of Pharmacy, Chitkara University, Rajpura, Punjab-140401
Keywords
RP-HPLC, Rufinamide, API, Method Validation
Abstract
A simple, rapid, sensitive, cost effective, and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the stability testing of rufinamide. The proposed RP-HPLC method was developed on phenome-nex LunaR C-18 5μm,250 mm × 4.6 mm id. Column (at ambient temperature) and a mobile phase consisting of phosphate buffer: acetonitrile (60:40) was delivered at a flow rate of 1.0ml/ min. The analyte was detected by using a UV detector at the wavelength of 293 nm. The method was found to be linear over the concentration range of 50- 150 μgml-1 (r2=0.999). 30. The retention time of rufinamide was 4.717 min.
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How to Cite
Jitender Singh, Sonia Sangwan, Parul Grover, Lovekesh Mehta, Deepika Kiran, Anju Goyal. Analytical Method Development and Validation for Assay of Rufinamide Drug.
J. Pharm. Technol. Res. Manag.. 2023, 01, 191-203
