53a7bb0819fc9ef4713-5632 20260804041800341 chitu:chitu chitkarauniversitypublications@chitkara.edu.in WEB-FORM Journal of Pharmaceutical Technology, Research and Management JPTRM 23212217 23212225 10.15415/jptrm https://jptrm.chitkara.edu.in/ 05 15 2026 05 15 2026 13 2 10.15415/jptrm.2025.132 https://jptrm.chitkara.edu.in/2025/vol-13-no-02/ HPTLC Driven Analytical Approaches for Antidiabetic Pharmaceuticals: A Detailed Review Atul A. Shirkhedkar R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur (Dhule), Maharashtra, India. https://orcid.org/0000-0002-6508-4183 Amod S. Patil R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur (Dhule), Maharashtra, India. https://orcid.org/0000-0002-4897-8855 Gurbinder Kaur TIET-VT, Center of Excellence in Emerging Materials (CEEMS), Thapar Institute of Engineering and Technology, Patiala, Punjab, India. https://orcid.org/0009-0004-0618-9315 Tejas Raju Jorvekar R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur (Dhule), Maharashtra, India. Background: Diabetes mellitus is a chronic disease affecting a large number of populations across the world, and it also requires management using anti-diabetic drugs. Various drugs such as metformin, vildagliptin, saxagliptin, sitagliptin, rosiglitazone, glipizide, and their combinations are commonly used for the treatment. Numerous pharmaceutical formulations are available in the market in combination forms as well. In order to ensure the quality, appropriate dosage, and regulatory compliance of these formulations, accurate and reliable analytical methods are essential. Purpose: This review will provide comprehensive insight into HPTLC-based analytical approaches used for qualitative and quantitative analysis of anti-diabetic drugs in various pharmaceutical formulations. Methods: This review critically summarizes the literature reporting HPTLC methods developed for the analysis of anti-diabetic drugs. Key aspects such as HPTLC method development, mobile phase and stationary phase selection, detection methods, and optimization strategies are discussed. Method validation parameters such as robustness, sensitivity, reproducibility, and accuracy are mentioned. Results: The study demonstrated that the HPTLC method is a reliable and efficient analytical technique for the evaluation of anti-diabetic drugs due to its low cost, high reproducibility, minimal solvent consumption, and ability to analyze multiple samples simultaneously. For routine quality control and research applications, validated HPTLC methods have shown satisfactory performance. Conclusion: HPTLC represents itself as a valuable tool for the qualitative as well as quantitative assessment of anti-diabetic formulations. This detailed review provides guidance for researchers as well as analysts in selecting and optimizing appropriate HPTLC protocols for research and quality control purposes. It also highlights the potential for further advancements in HPTLC-based drug analysis. 05 15 2026 05 15 2026 48 59 10.15415/jptrm.2025.132003 https://jptrm.chitkara.edu.in/2025/hptlc-analysis-antidiabetic-pharmaceuticals-review/