Pharmacognostic Standardization of Biophytum Sensitivum Dc

Abstract

Biophytum sensitivum DC. (Family – Oxalidaceae) is a traditional drug used in treating various ailments especially in mental disorders. The present study was envisaged to establish pharmacognostic standards of the plant so that authentic plant can be selected for research purpose. Heavy metal content, microbial load, aflatoxins contamination and pesticide residues were also examined to substantiate the standardization data. Transverse section of the root was cut and showed the presence of root hair, cork, cortex, pericyclic region, vascular bundles and pith whereas the stem showed the presence of trichomes, cork, cortex, pericylic fibres, xylem and phloem. Alcohol soluble extractive value was slightly higher than water soluble extractive values. Total ash, water soluble ash, acid-insoluble ash and sulphated ash were found to be 8.80, 5.26, 1.90 and 0.35% w/w respectively. Foreign matter, loss on drying, swelling index and foaming index were found to be nil, 8.50% w/w, nil, and less than 100 ml respectively. Microbial load, heavy metals and pesticide residues complied with the limits as described by WHO. Flavonoids, tannins, saponins, proteins and amino acids were present in the plant.

  • Page Number : 31-40
  • Keywords
    Biophytum sensitivum, Heavy Metal Analysis, Microbial Load, Pesticide residue
  • DOI Number
    10.15415/jptrm.2017.51003
  • Authors
    • ManishaDepartment of Pharmacognosy and Phytochemistry, Hindu College of Pharmacy, Sonipat-131001, India
    • Kumar SureshDepartment of Pharmaceutical Sciences and Drug research, Punjabi University, Patiala-147002, India

References

  • Anonymous (2015a). Guidelines for the appropriate use of herbal medicines, Western pacific series no. 23. Available from: http://apps.who.in. Accessed on 20 November, 2015.
  • Ariyanathan, S., Saraswathy, A. & Rajamanickam, G. V. (2010) . Quality control standards for the roots of three plumbago species. Indian Journal of Pharmaceutical Sciences, 72(1), 86–91. https://doi.org/10.4103/0250-474X.62254
  • Kirtikar, K. R. & Basu, B. D. (2003). Indian medicinal plants. Dehradun, India: International Book Distributors.
  • Anonymous (2015b). Enclyclopedia of Indian medicinal plants, herbs - medicinal plants usage and identification data base. Available from: http://herbs.indianmedicinalplants.info/index.php/sanskrit-names-ofplants/36-2012-02-18-18-09-08/151-biophytum-sensitivum. Accessed on 20 November, 2015.
  • Yun-Lian, L. & Wan-Yi, W. (2003). Chemical constituents of Biophytum sensitivum. Chinese Pharmaceutical Journal, 55(2), 71–75.
  • Ravishankara, M. N., Pillai, A. D., Padh, H. & Rajani, M. (2003). A sensitive HPTLC method for estimation of amentoflavone, a bioactive principle from Biophytum sensitivum (Linn.) DC. and Putranjiva roxburghii Wall. Journal of Planar Chromatography–Modern TLC, 16(3), 201-205. https://doi.org/10.1556/JPC.16.2003.3.6
  • Bucar, F., Jachak, S. M., Kartnig, T. & Schubert-Zsilavecz, M. (1998). Phenolic compounds from Biophytum sensitivum. Pharmazie, 53(4), 651–653.
  • Puri, D. & Baral, N. (1998). Hypoglycemic effect of Biophytum sensitivum in alloxan diabetic rabbits. Indian Journal of Physiology and Pharmacology, 42(3), 401–406.
  • Puri, D. (2001). The insulin tropic activity of a Nepalese medicinal plant Biophytum sensitivum: Preliminary experimental study. Journal of Ethnopharmacology, 78(1), 89–93. https://doi.org/10.1016/S0378-8741(01)00306-3
  • Puri, D. (2006). Screening of mildly hypoglycaemic compounds: Obese British Angora rabbits with borderline glucose intolerance as animal model. Indian Journal of Pharmaceutical Sciences, 68(5), 579–583. https://doi.org/10.4103/0250-474X.29623
  • Guruvayoorappan, C. & Kuttan, G. (2007). Immunomodulatory and antitumor activity of Biophytum sensitivum extract. Asian Pacific Journal of Cancer Prevention, 8(1), 27–32.
  • Puri, D. (2003). Hypocholesterolemic effect of Biophytum sensitivum leaf water extract. Pharmaceutical Biology, 41(4), 253–8. https://doi.org/10.1076/phbi.41.4.253.15680
  • Guruvayoorappan, C., Afira, A. H. & Kuttan, G. (2006). Antioxidant potential of Biophytum sensitivum extract in vitro and in vivo. Journal of Basic and Clinical Physiology and Pharmacology, 17(4), 255–267. https://doi.org/10.1515/JBCPP.2006.17.4.255
  • Jachak, S. M., Bucar, F. & Kartnig, T. (1999). Anti-inflammatory activity of extracts Biophytum sensitivum in carrageen-induced rat paw oedema. Phytotherapy Research, 13(1), 73–74. https://doi.org/10.1002/(SICI)1099-1573(199902)13:1<73::AID-PTR374>3.0.CO;2-V
  • Bhaskar, V. H. & Rajalakshmi, V. (2010). Anti-tumor activity of aqueous extract of Biophytum sensitivum Linn. Annals of Biology Research, 1(1), 76–80.
  • IP (2007). Indian Pharmacopoeia, Government of India, Ministry of Health and Family Welfare. New-Delhi, India: Controller of Publication.
  • Khandelwal, K. R. (2006). Practical pharmacognosy techniques and experiments. Pune, India: Nirali Prakashan.
  • Kokate, C. K. & Gokhale S. B. (2008). Practical pharmacognosy. Pune, India, Nirali Prakashan.
  • Chase, C. R. & Pratt R. S. (1949). Fluorescence of powdered vegetable drugs with particular reference to development of a system of identification. Journal of Pharmaceutical Sciences, 38(6), 324–331. https://doi.org/10.1002/jps.3030380612
  • Kokoshi, C. J., Kokoshi, R. J. & Slama, F. J. (1958). Fluorescence of powdered vegetable drugs under ultraviolet radiation. Journal of Pharmaceutical Sciences, 47(10), 715–717. https://doi.org/10.1002/jps.3030471010
  • WHO (1998). Quality control methods for medicinal plant materials. Geneva: World Health Organization Press.
  • Harborne, J. B. (1988). Phytochemical methods: A guide to modern techniques of plant analysis. London: Champan and Hall Publishing House. https://doi.org/10.1016/00319422(88)84139-6
  • Brain, K. R. & Turner T. D. (1975). The practical evaluation of phyto-pharmaceuticals, Bristol: Wright-Scientechnica Publishing House.

  • Published Date : 2017-05-05