Toxicological Evaluation of Crude Alkaloid Fraction Isolated From Indian Folklore Plant Telosma Pallida (Roxb) wg Craib Root Using Probit Value Analysis

Abstract

Present study first time reported the toxicological profile of Telosma pallida (TP) crude alkaloid fraction (CAF) isolated from the root of the climber plant by brine shrimp lethality test (BLT). Telosma pallida is a perennial herb found throughout the Junagadh district and surrounding. Previous studies showed the use of these alkaloids in the inhibition of thymidylate synthetase enzymes and cell growth. Brine shrimp toxicity study was carried out with Artemia salina Leach. In this assay, brine shrimp was hatched in sea salt water and allowed to contact with various concentrations of the crude alkaloid fractions. At 500μg/ml, highest mortality was found 74.44±0.35%whereas in the case of positive control, at the dose of 50μg/ml, 80.00±0.609% mortality was found. LD50 values for CAF and test control was found to be 89.12μg/ml and 12.59μg/ml, respectively. Further in-vitro and in-vivo studies may testify the anticancer potential of this plant.

  • Page Number : 139-146
  • Keywords
    Pergularia pallida, alkaloids, anticancer, brine shrimp, toxicity
  • DOI Number
    10.15415/jptrm.2016.42009
  • Authors
    • Punit R. BhattDepartment of Pharmacognosy, Shree Swaminarayan Pharmacy College, Kevadia Colony, Dist. Narmada (Gujarat) India
    • Kajal B. PandyaDepartment of Pharmacognosy, Shree Swaminarayan Pharmacy College, Kevadia Colony, Dist. Narmada (Gujarat) India
    • Navin R ShethGujarat Public Service Commission, Ahmedabad (Gujarat) India

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  • Published Date : 2016-11-02