In-Vitro Anti-oxidant And Antimicrobial Study of Ficus Hispida
Abstract
Ficus hispida L. belongs to the Moraceae family and is used by the maaiba trible (indigenous medicine - man of Manipur, India) as an indigenous traditional medicine. Present study deals with the successive extraction of the aerial parts of Ficus hispida and in-vitro screening of anti-oxidant and anti-microbial activity. The phytochemical screening of the methanol extract of Ficus hispida shows the presence of secondary metabolite groups like alkaloid, phenolic compounds, flavonoid, glycosides, protein etc. Phenolic compounds are commonly found in both edible and nonedible plants and are responsible for various medicinal activities of plants, so our study is based on determining antioxidant activity and anti-microbial activity. Beside these, we also measured the total flavonoid and total phenolic content of the respective sample to understand the effect of polyphenolic compound on different pathophysiological state associated with high free radical production. The in-vitro investigation proves the efficiency of this plant in various diseases states.
- Page Number : 153-166
- Keywords
Ficus hispida, Antioxidant activity, Minimum Inhibitory Concentration, Antimicrobial activity - DOI Number
10.15415/jptrm.2015.32012 -
Authors
- Atanu ChatterjeeBengal School of Technology (A College of Pharmacy), Sugandha, Delhi Road, Hooghly-712102, West Bengal, India
- Jayita MondalBengal School of Technology (A College of Pharmacy), Sugandha, Delhi Road, Hooghly-712102, West Bengal, India
- Rudranil BhowmikBengal School of Technology (A College of Pharmacy), Sugandha, Delhi Road, Hooghly-712102, West Bengal, India
- Anshuman BhattachayraBengal School of Technology (A College of Pharmacy), Sugandha, Delhi Road, Hooghly-712102, West Bengal, India
- Hirak RoyBengal School of Technology (A College of Pharmacy), Sugandha, Delhi Road, Hooghly-712102, West Bengal, India
- Swarnendu KunduBengal School of Technology (A College of Pharmacy), Sugandha, Delhi Road, Hooghly-712102, West Bengal, India
References
- Arteel, G.E., (2009). Oxidants and antioxidants in alcohol-induced liver disease. Gastroenterology, 124: 778–790. http://dx.doi.org/10.1053/gast.2003.50087
- Balasundram, S., Sundram, K., Sammar, S., (2006).Phenolic compounds of plants and agricultural by-products, antioxidant activity, Occurrence and potential uses. Journal of Food Chemistry, 68:191-203. http://dx.doi.org/10.1016/j.foodchem.2005.07.042
- Blois, M.S., (1958). Antioxidant determinations by the use of a stable free radical, Nature. 29:1199 1200. http://dx.doi.org/10.1038/1811199a0
- Davlo,s M., Rolnaldo, N., (1998).Alcoholic hepatitis. Review on Gastroenterology Peru, 18: 151–164. (1998).
- Ghosh, R., Sharotchandra, RS., (2004) Hypo glycemic activity of Ficus hispida in normal and diabetic albino rats. Indian Journal of Pharmacology 36: 222-225
- Gillman, B., Papachristodouluo, D.K., Thomas, J.H., Will’s Bio-chemical Basis of Medicine, 3rd Edition, Oxford. Butterworth Heinemann. p. 343
- Green, LC., Wagner, DA., Glogowski, J., (1982). Analysis of nitrate, nitrite and nitrate in biological fluids. Analytical Biochemistry, 126: 131–138. http://dx.doi.org/10.1016/0003-2697(82)90118-X
- Hatano, T., Edamaysu, R., Mori, A., Fujita, Y., Yasuhara, E., (1989). Chemistry and Pharmaceutical Bulletin, 37: 2016-2021. http://dx.doi.org/10.1248/cpb.37.2016
- Hsu, C., (2006).Antioxidant activity of extract from Polygonum aviculare L, Biological Research, 39, 281-288. http://dx.doi.org/10.4067/S0716-97602006000200010
- Hu, J., Lee, SO., Hendrich, S., Murphy, PA., (2002).Quantification of the group B soya saponins by high-performance liquid chromatography. Journal of Agricultural and Food Chemistry, 50: 2587–2594. http://dx.doi.org/10.1021/jf0114740
- Kahkonen, MP., Hopia, AI., Vuorela, HJ., Rauha, JP., PIhlaja, K., Kulaja, TS., (1999). Antioxidant activity of plant extracts containing phenolic compounds, Journal of Food Chemistry, 47:3954-3962. http://dx.doi.org/10.1021/jf990146l
- Khandelwal, K.R., (2012). Practical Pharmacognosy, 22nd edition, 23.10-23.11
- Kokate, C.K., Purohit, A.P., Gokhale, S.B., Book of Pharmacognosy, 46th edition, 8.57-8.58
- Kokate, C.K., Purohit, A.P., Gokhale, S.B., Book of Pharmacognosy, 46th edition, A.1-
- Kone, WM., Kamanzi, K., Traore, DA., Terreaux, C., Hostettmann, K., Dosso, M., (2004). Traditional medicine in North Cote-d’Ivoire screening of 50 medicinal plants for antibacterial activity. Journal of Ethnopharmacology; 93:43-49. http://dx.doi.org/10.1016/j.jep.2004.03.006
- Mandal, S.C., Ashok Kumar, C.K., (2002) Studies on anti-diarrhoeal activity of Ficus hispida leaf extract in rats. Fitoterapia, 73(7-8):663-7. http://dx.doi.org/10.1016/S0367-326X(02)00225-3
- Mandal, SC., Saraswathi B, Kumar, CK., Mohana Lakshmi, S., Maiti, BC., (2000)Protective effect of leaf extract of Ficus hispida Linn. Against paracetamol-induced hepatotoxicity in rats. Phytotherapy Research, 14(6):457-9. http://dx.doi.org/10.1002/1099-1573(200009)14:6<457::AID-PTR610>3.0.CO;2-8
- Marcocci, L., Maguire, JJ., Droy-Lefaix, MT., Packer, L., (1994a). The nitric oxide scavenging properties of Ginkgo biloba extract EGb 761. Biochemistry Biophysics Research Commuication, 15:748-755. http://dx.doi.org/10.1006/bbrc.1994.1764
- Marcocci, L., Packer, L., Droy-Lefaix, MT., Packer, L., (1994b). Antioxidant action of Ginkgo biloba extract EGB 761, Methods Enzymology, 234: 462-475. http://dx.doi.org/10.1016/0076-6879(94)34117-6
- Nadkarni, A. K., (1976). Materia Medica. (Pp.1031–1035). Popular Prakashan. Mumbai India.
- Rastogi, Mehrotra, BN., (1993). Compendium of Indian Medicinal Plants, Central Drug Research Institute; (pp. 27–30). Publication and Information Directorate, II. Lucknow, New Delhi.
- Ripu, M., Kunwar, I., Rainer, W. B., (2006) Ficus (Fig) species in Nepal a review of diversity and indigenous uses. Journal of Ecological Application,11:85–87.
- Ruch, R.J., Cheng, S.J., Klaunig, J.E., (1989). Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10, pp. 1003-1008. http://dx.doi.org/10.1093/carcin/10.6.1003
- Salah, N., Miller, NJ., Paganga, J., Tijburg, L., Bolwell, GP., Rice-Evans, C., (1995). Polyphenolic flavonols as scavengers of aqueous phase radicals and chain-breaking antioxidants. Archives of Biochemistry and Biophysics; 322:339-346. http://dx.doi.org/10.1006/abbi.1995.1473
- Sergio, R., Peraza, S., (2002)Constituents of leaves and twigs of Ficus hispida. Plant Med. 68: 186-188. http://dx.doi.org/10.1055/s-2002-20257
- Shanmugarajan, TS., Arunsunda, MC., (2008).Cardio protective effect of Ficus hispida Linn on cyclophosphamide provoked oxidative myocardial injury in a rat model. International Journal of Pharmacology, 1-10.
- Singh, H.K., Dhawan, B.N., (1997). Neuro-psychopharmacological effects of the Ayurvedic nootropic Bacopa monnieri Linn. (Brahmi). Indian Journal of Pharmacology, 29:S359- S365.
- Singleton, V L., Orthofer, L., Lamuela, Raventos., Rosa, M., (1999) Analysis of total phenol and other oxidation substrate and antioxidant by means of folin-ciocalteu reagents, Methods in Enzymology, 299, 152-178. http://dx.doi.org/10.1016/S0076-6879(99)99017-1
- Strube, M., Dragstedt, LO., Larsen, JC., (1993).Naturally occurring Anti-tumourigens, Plant Phenols, The Nordic Councils of Ministers, Copenhagen, 39-40.
- Sun, J., Chu, Y. F., Wu, X. Z., Liu, R. H., (2002) Journal of Agriculture and Food Chemistry, 50:7449 -7454. http://dx.doi.org/10.1021/jf011617i
- Tsao, R., Akhtar, M.H., (2005). Nutraceuticals and functional foods: I. Current trend in phytochemical antioxidant research. Journal of Food, Agriculture and Environment, 3(1): 10-17.
- Vishnoi, SP., Jha, T., (2004) Evaluation of anti-inflammatory activity of leaf extracts of Ficus hispida. Indian Journal of Natural Product, 20:27–29.
- Yoshiki, K., Kudou, S., Okubo, K., (1998).Relationship between chemical structures and biological activities of triterpinoid saponins from soyabean. Bioscience, Biotechnology, and Biochemistry, 62: 2291-2299. http://dx.doi.org/10.1271/bbb.62.2291
- Yoshiki, Y., Okubo, K., (1995). Active oxygen scavenging activity of DDMP (2, 3-dihydro-2, 5-dihydrox saponin in soybean seed. Bioscience, Biotechnology, and Biochemistry, 59: 1556–1557. http://dx.doi.org/10.1271/bbb.59.1556
- Zheng, W., Wang, S. Y., (2001) Journal of Agriculture and Food Chemistry, 49:5165-5170. http://dx.doi.org/10.1021/jf010697n
Published Date : 2015-11-17