UV-Spectrophotometry - Multicomponent Mode of Analysis for Simultaneous Estimation of Brinzolamide and Brimonidine Tartrate in Bulk and Ophthalmic Formulation

Abstract

Brinzolamide (BRZ) and Brimonidine Tartrate (BT) in combination are available as an ophthalmic suspension in the ratio of 5:1. A simple, reproducible and efficient method for the simultaneous determination of BRZ and BT in Bulk and Ophthalmic formulation has been developed. The absorbance was assessed at two wavelengths i.e. 252.40 nm (λ max of BRZ) and 246 nm (λmax of BT) in methanol. In this method, BRZ and BT executed linearity in the concentration range of 5-35 μg/mL and 3-18 μg/mL, respectively at their respective λ max. The developed method was found to be accurate, precise and rugged as marked by small values of % RSD according to ICH guidelines.

• Page Number : 31-35
• Keywords
  Brinzolamide, Brimonidine tartrate, UV-Spectrophotometry, Multicomponent mode of analysis, Validation
• DOI Number
  https://doi.org/10.15415/jptrm.2019.71005
• Authors
  
  • Vikram L. Jadhav Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist.: Dhule (M.S.) India 425 405
  • Amod S. Patil Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist.: Dhule (M.S.) India 425 405
  • Suraj R. Chaudhari Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist.: Dhule (M.S.) India 425 405
  • Atul A. Shirkhedkar Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist.: Dhule (M.S.) India 425 405