Estimation of Total Phenols and Flavonoids in Selected Indian Traditional Plants

  • Deepak Kumar Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147 002, Punjab, India
  • Anupam Jamwal Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147 002, Punjab, India
  • Reecha Madaan Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab-140401
  • Suresh Kumar Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147 002, Punjab, India
Keywords: Abies pindrow, Abies webbiana, Calotropis gigantea, Cephalandra indica, Flavonoids, Phenols

Abstract

Traditionally, aerial parts of Abies pindrow Royle (Himalayan Fir; Pinaceae); Abies webbiana Lindl. (Talispatra; Pinaceae); Cephalandra indica Naud. (Ivy Gourd; Cucurbitaceae) and roots of Calotropis gigantea (L.) Dryand (Giant Milkweed; Asclepiadaceae) have been used in the Indian systems of medicine for the treatment of various ailments. But no systematic phytochemical work has ever been carried out on these potential plants. Thus, it was planned to estimate total phenols and flavonoids content in methanol extract, ethyl acetate fraction (EAF) and remaining methanol extract (RME) of selected plants. Properly identified plants were defatted with petroleum ether, and then separately extracted in a Soxhlet apparatus with methanol. The methanol extract of each plant was partitioned by ethyl acetate solvent to obtain EAF of respective plant. The total phenols and flavonoids contents were estimated using standardized procedures. Quantitative determination of total phenols and total flavonoids was done using standard curve of gallic acid (linearity: 20 to 120 mg/ml; r2 = 0.995) and quercetin (linearity: 30 to 180 mg/ml; r2 = 0.997), respectively. EAF of selected plants contained higher content of phenols and flavonoids, where as lowest content was observed in RME of plants. The content of total phenols and flavonoids in selected plants were found to be in order of C. indica > A. webbiana > A. pindrow > C. gigantea. The available literature reveals that polyphenols have been reported to possess varied pharmacological activities. As selected Indian plants contain polyphenols as major class of phytoconstituents, it is suggested that these constituents may be responsible for their medicinal uses.

Downloads

Download data is not yet available.

References

Chandira, M., Vankateswarlu, B.S., Gangwar, R.K., Sampathkumar, P., Bhowmik, D., Jayakar, B. & Rao, C.V. (2010). Studies on anti-stress and free radical scavenging activity of whole plant of Coccinia indica Linn. International Journal of Pharmaceutical Sciences, 1, 50-54. http://dx.doi.org/10.13040/IJPSR.0975-8232.

Chopra, R.N., Nayar, S.L. & Chopra, I.C. Glossary of Indian Medicinal Plants, New Delhi, India: Council of Scientific and Industrial Research, 1956.

Dubey, N., Kumar, R. & Tripathi, P. (2004). Global promotion of herbal medicine: India’s opportunity. Current Science, 86, 37-41.

Duke, J.A., Duke, P.A. & Ducellier, J.L. Duke’s Handbook of Medicinal Plants of the Bible, New York: CRC Press, 2008.

Farnsworth, N.R. (1966). Biological and phytochemical screening of plants. Journal of Pharmaceutical Sciences, 55, 225-276. http://dx.doi.org/10.1002/jps.2600550302.

Fernandez, S.P., Wasowski, C., Loscalzo, L.M., Granger, R.E., Johnston, G.A.R., Paladini, A,C. & Marder, M. (2006). Central nervous system depressant action of flavonoid glycosides. European Journal of Pharmacology, 539, 168-176. http://dx.doi.org/10.1016/j.ejphar.2006.04.004.

Gupta, A.K. & Sharma, M. Reviews on Indian Medicinal Plants, New Delhi, India: Council of Medical Research, 2007.

Haramoto, M., Tatemoto, H. & Muto, N. (2008). Essential role of ascorbic acid in the neural differentiation and developed: High levels of ascorbic acid 2-glucoside effectively enhance nerve growth factor-induced neurite formation and elongation of PC 12 cells. Journal of Health Science, 54, 43-49.

Hotta, H., Nagano, S., Ueda, M., Tsujino, Y., Koyama, J. & Osakai, T. (2002). Higher radical scavenging activity of polyphenolic antioxidants ascribed to chemical reactions following oxidation. Biochimica et Biophysica Acta, 1572, 123-132. http://dx.doi.org/10.1016/S0304-4165(02)00285-4.

Iyengar, M.A., Bhat, K.G., Nayak, S.G.K., Rajgopal, P.K. & Nanada, A. (1986). Survey of medicinal flora of South Kanara, Series I. Indian Drugs, 24, 69-73.

Jain, S.K. & Tarafder, C.R. (1970). Medicinal plants-lore of the Santals. (A revival of P.O. Bodding’s work). Economic Botany, 24, 241-278. http://dx.doi.org/10.1007/BF02860661.

Jha, R.K. (2001). Ethnomedicinal plants used against “Asthma” at Chhotanagpur, Jharkhand. Advances in Plant Sciences, 14, 95-97.

Katewa, S.S., Chaudhary, B.L., Jain, A. & Galav, P. (2003). Traditional uses of plant biodiversity from Aravalli hills of Rajasthan. Indian Journal of Traditional Knowledge, 2, 27-39.

Khare, C.P. Indian Medicinal Plants: An Illustrated Dictionary, New York: Spinger Science and Business Media, 2007.

Kirtikar, K.R. & Basu, B.D. Indian Medicinal Plants, Allahabad, India: International Book Distributors, 1975.

Lee, S.E., Hwang, H.J., Ha, J.S., Jeong, H.S. & Kim, J.H. (2003). Screening of medicinal plants extracts for antioxidant activity. Life Sciences, 73, 167-179. http://dx.doi.org/10.1016/S0024-3205(03)00259-5.

Madaan, R., Bansal, G., Kumar, S. & Sharma, A. (2011). Estimation of total phenols and flavonoids in extracts of Actaea spicata roots and antioxidant activity studies. Indian Journal of Pharmaceutical Sciences, 73, 666-669. http://dx.doi.org/10.4103/0250-474X.100242.

Marinova, D., Ribarova, F. & Atanassova, M. (2005). Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of Chemical Technology and Metallurgy, 40, 255-260.

Saaby, L., Rasmussen, H.B. & Jager, A.K. (2009). MAO-A inhibitory activity of quercetin from Calluna vulgaris (L.) Hull. Journal of Ethnopharmacology, 121, 178-181.

http://dx.doi.org/10.1016/j.jep.2008.10.012.

Sharma, A., Shanker, C., Tyagi, L.K., Singh, M. & Rao, V. (2008). Herbal medicine for market potential in India: An overview. Academic Journal of Plant Sciences, 1, 26-36.

Skerget, M., Kotnik, P., Hadolin, M., Hras, A.R., Simonic, M. & Knez, Z. (2005). Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chemistry, 89, 191-198. http://dx.doi.org/10.1016/j.foodchem.2004.02.025.

Sulaiman, C.T. & Balachandran, I. (2012). Total phenolics and total flavonoids in selected Indian medicinal plants. Indian Journal of Pharmaceutical Sciences, 74, 258-260.

http://dx.doi.org/10.4103/0250-474X.106069.

Viola, H., Wasowski, C., Destein, M.L., Wolfman, C., Silveira, R., Dajas, F., Medina, J.H. & Paladini, A.C. (1995). Apigenin, a component of Matricaria recutita flowers, is a central benzodiazepine receptors ligand with anxiolytic effects. Planta Medica, 61, 213-216. http://dx.doi.org/10.1055/s-2006-958058.

Published
2014-05-07
How to Cite
Deepak Kumar, Anupam Jamwal, Reecha Madaan, & Suresh Kumar. (2014). Estimation of Total Phenols and Flavonoids in Selected Indian Traditional Plants. Journal of Pharmaceutical Technology, Research and Management, 2(1), 77-86. https://doi.org/10.15415/jptrm.2014.21006
Section
Articles