Neuropharmacological Profile of Fractions of Actaea acuminata H. Hara Roots

  • Deepak Kumar Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147 002, Punjab, India
  • Suresh Kumar Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147 002, Punjab, India
Keywords: Actaea acuminata,, Alkaloids, Flavonoids, Himalayan Baneberry

Abstract

The methanol extract of Actaea acuminata roots have beenpreviously reported to exhibit significant antianxiety, anticonvulsant andantidepressant activities. In present study fractionation of crude bioactivemethanol extract was carried out using different solvents employing standardprocedure. Various fractions were evaluated for antianxiety, anticonvulsantand antidepressant activities using elevated plus maze model (EPM), maximalelectroshock-induced convulsions model (MES) and forced / despair swim test(FST) respectively. Successive partitioning of bioactive methanol extract wasdone with ethyl acetate and 1-butanol. The ethyl acetate fraction (EAF; 100mg/kg), 1-butanol fraction (BF; 25 mg/kg) and remaining methanol extract(RME; 70 mg/kg) were subjected to neuropharmacological activities. TheEAF significantly enhanced entries and average time spent in open arms;significantly decreased duration of MES-induced tonic extension phase andsignificantly decreased duration of immobility time of rats in comparisonto control. BF and RME did not exhibit any neuropharmacological activity.Qualitative chemical tests confirmed presence of alkaloids and polyphenolsin EAF. It is finally concluded that alkaloids and/or polyphenols are bioactiveconstituents of A. acuminata which are responsible for neuropharmacologicalactivities.

References

Anonymous. (1948). A Dictionary of Indian Raw Materials and Industrial Products, in the Wealth of India. Council of Scientific and Industrial Research, New Delhi, India, 314.

Belzung, C. & Griebel, G. (2001). Measuring normal and pathological anxiety like behaviour in mice: A review. Brain Research Reviews, 125(1-2), 141–149. https://doi.org/10.1016/S0166-4328(01)00291-1

Biswas, D., Kumar, D. & Kumar, S. (2017). Neuropharmacological investigations on Actaea acuminata H. Hara roots. Indian Journal of Experimental Biology, 55(1), 27–35.

Chopra, R.N., Nayar, S.L. & Chopra, I.C. (1956). Actaea acuminata, in Glossary of Indian Medicinal Plants. Council of Scientific and Industrial Research, New Delhi, India, 7.

Farnsworth, N.R. (1966). Biological and phytochemical screening of plants. Journal of Pharmaceutical Sciences, 55(3), 225–276. https://doi.org/10.1002/jps.2600550302

Khare, C.P. (2007). Actaea spicata Linn., in Indian Medicinal Plants: An Illustrated Dictionary. Spinger Science and Business Media, New York, USA, 17.

Kirtikar, K.R. & Basu, B.D. (1975). Actaea spicata Linn., in Indian Medicinal Plants. International Book Distributors, Allahabad, India, 51.

Mcdaid, D., Knapp, M. & Raja, S. (2008). Barriers in the mind: Promoting an economic case for mental health in low and middle income countries. World Psychiatry, 7(2), 79–86. https://doi.org/10.1002/j.2051-5545.2008.tb00160.x

NAMI. (2013). Mental Health Issues are Prevalent on College Campuses. National Alliance on Mental Illness. Available from: https://www.nami.org/Press-Media/In-The-News/2017/How-Parents-CanIdentify-Mental-Health-Problems-in. Accessed on July, 2017.

NIH. (2008). NIH Consensus Development Conference Statement on Management of Mental Heath. National Institutes of Health. Available online: https://medlineplus.gov/ mentaldisorders.html. Accessed on July, 2017.

Prince, M., Patel, V., Saxena, S., Maj, M., Maselko, J., Phillips, M.R. & Rahman, A. (2007). No health without mental health. The lancet, 370(9590), 859 877. https://doi.org/10.1016/S0140-6736(07)61238-0

Rockville. (1999). U.S. Department of Health and Human Services. Substance Abuse and Mental Health Services Administration. Center for Mental Health Services, Maryland, 411.

Scheffer, W.C. (1980). Statistics for the Biological Sciences. Addison-Wesley Publishing Company, Philippines, 121-141.

Swinyard, E.A., Brown, W.C. & Goodman, L.S. (1952). Comparative assays of antiepileptic drugs in mice and rats. Journal of Pharmacology and Experimental Therapeutics, 106(3), 319–330.

Weiss, J.M., Goodman, P.A., Losito, G.O., Corrigan, S., Carris, J.M. & Bailey, W.H. (1981). Behavioural depression produced by uncontrollable stressor: Relationship to norepinephrine, dopamine and serotonin levels in various regions of rat brain. Brain Research Reviews, 3(2), 167–205. https://doi.org/10.1016/0165-0173(81)90005-9

Published
2018-05-02
How to Cite
Deepak Kumar, & Suresh Kumar. (2018). Neuropharmacological Profile of Fractions of Actaea acuminata H. Hara Roots. Journal of Pharmaceutical Technology, Research and Management, 6(1), 1-8. Retrieved from https://jptrm.chitkara.edu.in/index.php/jptrm/article/view/7
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Articles