Standardization of Some Commercial Anti-diabetic Herbal Products containing Syzygiumcumini
An isocratic HPLC method was developed for standardization of five commercially available products containing S. cumini seeds (DIABECON, MADHULENE, HYPONIDD, D-FIT and DIABEGON)using methylxanthoxylin (MXX)as marker. The method was validated in accordance with ICH Q2(R1) guidelines. The chromatographic separation of MXX was achieved on a C18 column by mobile phase composed of methanol and water (60:40%v/v) at a flow rate 0.5 mL/min. The eluent was detected at 280 nm.
The method was linear over concentration of 1-200 μg/mL with correlation coefficient of 0.9999. The LOD and LOQ were 0.175 and 0.530 µg/mL, respectively. The method was precise (%RSD <0.31), accurate and robust for determination of MXX in herbal extracts. The content of MXX in the seed extract was found to be 0.0433%w/w while it was ranging from 0.026-
0.041%w/w in the products. The content of MXX was found to be equivalent to the pure seed extract only in DIABECON tablets and D-FIT soft gelatine capsules while it was found to be significantly less in the other formulations.
Brito, F.A., Lima, L.A., Ramos, M.F., Nakamura, M.J., Cavalher-Machado, S.C., et al. (2007)’Pharmacologicalstudy of anti-allergicactivity of Syzygium cumini (L.) Skeels’.
Brazilian Journal of Medical and Biological Research, Vol 40, 1, pp. 105-115. https://doi.org/10.1590/S0100-879X2007000100014
Chaturvedi, A., Kumar, M.M., Bhawani, G., Chaturvedi, H., Kumar, M., et al. (2007)’Effect of ethanolic extract of Eugenia jambolana seeds on gastric ulceration and secretion in rats’. Indian Journal of Physiology and Pharmacology, Vol 51, 2, pp. 131-140.
Gupta, R. and Saxena, A.M. (2011)’Hypoglycemic and Anti-hyperglycemic Activities of Syzygium cumini (Linn.) Skeels Whole Fruit, in Normal and Streptozotocin-Induced Diabetic Rats’. Asian Journal of Pharmaceutical and Biological Research, Vol 1, 3, pp. 2231-2218.
International Conference on Harmonization (2005) Validation of analytical procedures: Text and methodologyICH Q2(R1), International Conference on Harmonization, Geneva, Switzerland.
Kaneria, M. and Chanda, S. (2013)’Evaluation of antioxidant and antimicrobial capacity of Syzygium cumini L. leaves extracted sequentially in different solvents’. Food Chemistry, Vol 37, 2, pp. 168-176. https://doi.org/10.1111/j.1745-4514.2011.00614.x
Kavitha, K., Murali, M. and Jayachandra, K. (2011) ‘Preliminary Phytochemical Screening, Anthelmintic Activity of Methanolic and Aqueous Extract of Syzygium Cumini Linn.
Bark (Myrtaceae)’. Journal of Pharmaceutical Sciences and Research, Vol 3, 9, pp. 1460-1465.
Kumar, A., Ilavarasan, R., Jayachandran, T., Deecaraman, M., Kumar, R.M., et al. (2008)’Antiinflammatory activity of Syzygium cumini seed’. African Journal of Biotechnology, Vol 7, 8, pp. 941-943.
Murti, K., Paliwal, D., Madan, S., Kundu, R. and Kaushik, M. (2012) ‘Exploration of PreliminaryPhytochemical Studies of Seed of Syzygium cumini’. American Journal of Pharmacology and Toxicology, Vol 7, 1, pp. 12-14. https://doi.org/10.3844/ajptsp.2012.12.14
Pareek, A. and Meena, R.K. (2015)’Antimicrobial Activity of Syzygium Cumini’. Indian Journal of Applied Research, 5(8), 751-753.
Ravi, K., Rajasekaran, S. and Subramanian, S. (2005)’Antihyperlipidemic effect of Eugenia jambolana seed kernel on streptozotocin-induced diabetes in rats’. Food and Chemical Toxicology, Vol 43, 9, pp. 1433-1439. https://doi.org/10.1016/j.fct.2005.04.004
Shamkuwar, P.B., Pawar, D.P. and Chauhan, S.S. (2012)’Antidiarrhoeal activity of seeds of Syzygium cumini L’. Journal of Pharmacy Research, Vol 5, 12, pp. 5537-5539.
Shinde, J., Taldone, T., Barletta, M., Kunaparaju, N., Hu, B., et al. (2008)’α-Glucosidase inhibitory activity of Syzygium cumini (Linn.) Skeels seed kernel in vitro and in Goto-Kakizaki (GK) rats’. Carbohydrate Research, Vol 343, pp. 1278-1281. https://doi.org/10.1016/j.carres.2008.03.003
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