UV-Spectrophotometry - Multicomponent Mode of Analysis for Simultaneous Estimation of Brinzolamide and Brimonidine Tartrate in Bulk and Ophthalmic Formulation
Brinzolamide (BRZ) and Brimonidine Tartrate (BT) in combination are available as an ophthalmic suspension in the ratio of 5:1. A simple, reproducible and efficient method for the simultaneous determination of BRZ and BT in Bulk and Ophthalmic formulation has been developed. The absorbance was assessed at two wavelengths i.e. 252.40 nm (λ max of BRZ) and 246 nm (λmax of BT) in methanol. In this method, BRZ and BT executed linearity in the concentration range of 5-35 μg/mL and 3-18 μg/mL, respectively at their respective λ max. The developed method was found to be accurate, precise and rugged as marked by small values of % RSD according to ICH guidelines.
 Indian Pharmacopoeia. Ministry of Health and Family Welfare, Government of India, the Indian Pharmacopoeial Commission, Ghaziabad, 2, 2014, 1201–1202.
 THE MERCK INDEX-An encyclopedia of Chemicals, Drugs, And Biologicals, 13th edition, MERCK & CO., INC. Whitehouse Station, NJ, 2001,1361–1362.
 Shah, P. A., Kadikar, A. S., Katira, R. M., Patel, K. G., and Gandhi, T. R. (2014). Simultaneous determination of Brinzolamide and Timolol maleate using three different spectrophotometric methods. World Journal of Pharmacy and Pharmaceutical Sciences, 3(2),1955-1967.
 Khatun, R., and Islam, S. A. (2014). Development and validation of analytical method for simultaneous estimation of Brinzolamide and Timolol by HPLC from ophthalmic preparation. International Journal of Pharmaceutical Sciences and Research, 5(3), 1001.https://doi.org/10.13040/IJPSR
 Jain, P. S., Khatal, R. N., &Surana, S. J. (2011). Development and Validation of First Order Derivative UV-Spectrophotometric Method for Determination of Brimonidine tartrate in Bulk and in Formulation. Asian Journal of Pharmaceutical & Biological Research (AJPBR), 1(3).
 Shirke, R.R., and Pai, N. (2002). RP-HPLC determination of Brimonidine tartrate in Brimonidine tartrate eye drops. Indian drugs, 39(9), 484-486.
 Mahajan, A., Athensia, F., Gandhi, S. V., and Deshpande, P. B. (2010). Development and Validation of High-Performance Thin-Layer Chromatographic Method for Estimation of Brimonidine Tartrate as Bulk Drug and in Ophthalmic Solutions.International Journal Chemical Technology and Research, 2(3), 1376–1379.
 Jiang, S., Chappa, A. K., and Proksch, J. W. (2009). A rapid and sensitive LC/MS/MS assay for the quantitation of Brimonidine in ocular fluids and tissues. Journal of Chromatography B, 877(3),107–114.https://doi.org/10.1016/j.jchromb.2008.11.009
 Sonalis, M. C., and Rajput, A. P. (2011). Development and validation of a new stability-indicating analytical method for the determination of related components of Brimonidine tartrate in drug substances and drug product using UPLC, International Journal Pharmacy and PharmaceuticalScience, 3(1), 145–150.
 Phogat, A., Kumar, M. S., and Mahadevan, N. (2011). Simultaneous estimation of Brimonidine tartrate and Timolol maleate in nanoparticles formulation by RP-HPLC. Int J Recent Adv Pharm Res, 3, 31–36.
 Jain P. S., Khatal R. N., Jivani H. N., and Surana S. J. (2011). Development and Validation of TLC-densitometry Method for Simultaneous Estimation of Brimonidine tartrate and Timolol maleate in Bulk and Pharmaceutical Dosage Form. Journal Chromatography Separation Techniques,2(3), 1–5.https://doi.org/10.4172/2157-7064.1000113
 ICH Validation of analytical procedures: Text and methodology Q2 (R1), International Conference on Harmonization, 2005.
 Dhandar, A. G., Ganorkar, S. B., Patil, A. S., and Shirkhedkar, A. A. (2018). Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Quinfamide and Mebendazole in in-house Pharmaceutical Formulation, Journal of Pharmaceutical Technology, Research and Management6(1), 9–20.https://doi.org/10.15415/jptrm.2018.61002
 Patil, A. S., Shirkhedkar, A. A., Surana, S. J., and Nawale, P. S. (2011). Q-absorbance and multicomponent UV spectrophotometric methods for simultaneous estimation of propranolol hydrochloride and flunarizinedi hydrochloride in capsules. Der Pharma Chemica, 3(3), 404–408.
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