Exploring RP-HPLC Method for analysis of Axitinib in Bulk and in-house Tablets

  • Shailesh S Chalikwar R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur 425405 Dist. Dhule (MS), India
  • Satish D Kayande R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur 425405 Dist. Dhule (MS), India.
  • Inderbir Singh Chitkara College of Pharmacy, Chitkara University, Rajpura 140401, Patiala Punjab, India
  • Atul A Shirkhedkar R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur 425405 Dist. Dhule (MS), India
Keywords: Axitinib, HPLC, validation, in-house tablets

Abstract

Axitinib is a tyrosine kinase Inhibiter. In a commenced analysis, a effortless and responsive high-performance liquid-chromatography method was developed and validated for the quantitative estimation of Axitinib in bulk and in-house tablet dosage form. The present method was developed and validated using LC-GC Qualisil BDS C18 (250 mm × 4.6 mm, 5 μm). The separation of Axitinib was employed using a methanol: water 85:15% v/vas a mobile phase at optimal flow rate 1 mL/min and column oven temperature 30°C. While, Axitinib was examined at 330 nm with a photo diode array detector; retention timewas found to be 3.23 min.The intended method was validated by ICH rules for the accuracy, precision, sensitivity, and ruggedness. The linearity was followed in the concentration range of 4 - 24 μg/ mL as demonstrated by correlation coefficient (r2) of 0.9994. The robustness of proposed method was assessed by purposelyvarying the chromatographic conditions. Consequently, the intended method can routinely be subjected for th estimation of Axitinib in bulk and in tablets formulation.

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How to Cite
Shailesh S Chalikwar, Satish D Kayande, Inderbir Singh, & Atul A Shirkhedkar. (1). Exploring RP-HPLC Method for analysis of Axitinib in Bulk and in-house Tablets. Journal of Pharmaceutical Technology, Research and Management, 6(2), 135-141. Retrieved from https://jptrm.chitkara.edu.in/index.php/jptrm/article/view/103
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