J. Pharm. Technol. Res. Manag.

Neuropharmacological Profile of Fractions of Actaea acuminata H. Hara Roots

Deepak Kumar and Suresh Kumar

  • Download PDF
  • DOI Number
    https://doi.org/10.15415/jptrm.2018.61001
KEYWORDS

Actaea acuminata, Alkaloids, Flavonoids, Himalayan Baneberry.

PUBLISHED DATE May 2018
PUBLISHER The Author(s) 2018. This article is published with open access at www.chitkara.edu.in/publications
ABSTRACT

The methanol extract of Actaea acuminata roots have been previously reported to exhibit significant antianxiety, anticonvulsant and antidepressant activities. In present study fractionation of crude bioactive methanol extract was carried out using different solvents employing standard procedure. Various fractions were evaluated for antianxiety, anticonvulsant and antidepressant activities using elevated plus maze model (EPM), maximal electroshock-induced convulsions model (MES) and forced / despair swim test (FST) respectively. Successive partitioning of bioactive methanol extract was done with ethyl acetate and 1-butanol. The ethyl acetate fraction (EAF; 100 mg/kg), 1-butanol fraction (BF; 25 mg/kg) and remaining methanol extract (RME; 70 mg/kg) were subjected to neuropharmacological activities. The EAF significantly enhanced entries and average time spent in open arms; significantly decreased duration of MES-induced tonic extension phase and significantly decreased duration of immobility time of rats in comparison to control. BF and RME did not exhibit any neuropharmacological activity. Qualitative chemical tests confirmed presence of alkaloids and polyphenols in EAF. It is finally concluded that alkaloids and/or polyphenols are bioactive constituents of A. acuminata which are responsible for neuropharmacological activities.

Page(s) 1–8
URL http://dspace.chitkara.edu.in/jspui/bitstream/123456789/718/1/JPTRM_1_Thakur.pdf
ISSN Print : 2321-2217, Online : 2321-2225
DOI https://doi.org/10.15415/jptrm.2018.61001
REFERENCES
  • Anonymous. (1948). A Dictionary of Indian Raw Materials and Industrial Products, in the Wealth of India. Council of Scientific and Industrial Research, New Delhi, India, 314.
  • Belzung, C. & Griebel, G. (2001). Measuring normal and pathological anxiety like behaviour in mice: A review. Brain Research Reviews, 125(1-2), 141–149. https://doi.org/10.1016/S0166-4328(01)00291-1
  • Biswas, D., Kumar, D. & Kumar, S. (2017). Neuropharmacological investigations on Actaea acuminata H. Hara roots. Indian Journal of Experimental Biology, 55(1), 27–35.
  • Chopra, R.N., Nayar, S.L. & Chopra, I.C. (1956). Actaea acuminata, in Glossary of Indian Medicinal Plants. Council of Scientific and Industrial Research, New Delhi, India, 7.
  • Farnsworth, N.R. (1966). Biological and phytochemical screening of plants. Journal of Pharmaceutical Sciences, 55(3), 225–276. https://doi.org/10.1002/jps.2600550302
  • Khare, C.P. (2007). Actaea spicata Linn., in Indian Medicinal Plants: An Illustrated Dictionary. Spinger Science and Business Media, New York, USA, 17.
  • Kirtikar, K.R. & Basu, B.D. (1975). Actaea spicata Linn., in Indian Medicinal Plants. International Book Distributors, Allahabad, India, 51.
  • Mcdaid, D., Knapp, M. & Raja, S. (2008). Barriers in the mind: Promoting an economic case for mental health in low and middle income countries. World Psychiatry, 7(2), 79–86. https://doi.org/10.1002/j.2051-5545.2008.tb00160.x
  • NAMI. (2013). Mental Health Issues are Prevalent on College Campuses. National Alliance on Mental Illness. Available from: https://www.nami.org/Press-Media/In-The-News/2017/How-Parents-CanIdentify-Mental-Health-Problems-in. Accessed on July, 2017.
  • NIH. (2008). NIH Consensus Development Conference Statement on Management of Mental Heath. National Institutes of Health. Available online: https://medlineplus.gov/ mentaldisorders.html. Accessed on July, 2017.
  • Prince, M., Patel, V., Saxena, S., Maj, M., Maselko, J., Phillips, M.R. & Rahman, A. (2007). No health without mental health. The lancet, 370(9590), 859–877. https://doi.org/10.1016/S0140-6736(07)61238-0
  • Rockville. (1999). U.S. Department of Health and Human Services. Substance Abuse and Mental Health Services Administration. Center for Mental Health Services, Maryland, 411.
  • Scheffer, W.C. (1980). Statistics for the Biological Sciences. Addison-Wesley Publishing Company, Philippines, 121-141.
  • Swinyard, E.A., Brown, W.C. & Goodman, L.S. (1952). Comparative assays of antiepileptic drugs in mice and rats. Journal of Pharmacology and Experimental Therapeutics, 106(3), 319–330.
  • Weiss, J.M., Goodman, P.A., Losito, G.O., Corrigan, S., Carris, J.M. & Bailey, W.H. (1981). Behavioural depression produced by uncontrollable stressor: Relationship to norepinephrine, dopamine and serotonin levels in various regions of rat brain. Brain Research Reviews, 3(2), 167–205. https://doi.org/10.1016/0165-0173(81)90005-9