J. Pharm. Technol. Res. Manag.

Standardization of Some Commercial Anti-diabetic Herbal Products containing Syzygiumcumini

Jasmeen Kaur, Munish Singla, Balraj Saini And Gulshan Bansal*


Methylxanthoxylin, Syzygiumcumini, HPLC, stability testing, anti-diabetic.

PUBLISHER The Author(s) 2017. This article is published with open access at www.chitkara.edu. in/publications

An isocratic HPLC method was developed for standardization of five commercially available products containing S. cumini seeds (DIABECON, MADHULENE, HYPONIDD, D-FIT and DIABEGON)using methylxanthoxylin (MXX)as marker. The method was validated in accordance with ICH Q2(R1) guidelines. The chromatographic separation of MXX was achieved on a C18 column by mobile phase composed of methanol and water (60:40%v/v) at a flow rate 0.5 mL/min. The eluent was detected at 280 nm. The method was linear over concentration of 1-200 μg/mL with correlation coefficient of 0.9999. The LOD and LOQ were 0.175 and 0.530 µg/mL, respectively. The method was precise (%RSD <0.31), accurate and robust for determination of MXX in herbal extracts. The content of MXX in the seed extract was found to be 0.0433%w/w while it was ranging from 0.026- 0.041%w/w in the products. The content of MXX was found to be equivalent to the pure seed extract only in DIABECON tablets and D-FIT soft gelatine capsules while it was found to be significantly less in the other formulations.

Page(s) 21–30
URL http://dspace.chitkara.edu.in/jspui/bitstream/1/807/3/02_JPTRM_Gulshan.pdf
ISSN Print : 2321-2217, Online : 2321-2225
DOI 10.15415/jptrm.2017.51002
  • Brito, F.A., Lima, L.A., Ramos, M.F., Nakamura, M.J., Cavalher-Machado, S.C., et al. (2007)’Pharmacologicalstudy of anti-allergicactivity of Syzygium cumini (L.) Skeels’.
  • Brazilian Journal of Medical and Biological Research, Vol 40, 1, pp. 105-115. https://doi.org/10.1590/S0100-879X2007000100014
  • Chaturvedi, A., Kumar, M.M., Bhawani, G., Chaturvedi, H., Kumar, M., et al. (2007)’Effect of ethanolic extract of Eugenia jambolana seeds on gastric ulceration and secretion in rats’. Indian Journal of Physiology and Pharmacology, Vol 51, 2, pp. 131-140.
  • Gupta, R. and Saxena, A.M. (2011)’Hypoglycemic and Anti-hyperglycemic Activities of Syzygium cumini (Linn.) Skeels Whole Fruit, in Normal and Streptozotocin-Induced Diabetic Rats’. Asian Journal of Pharmaceutical and Biological Research, Vol 1, 3, pp. 2231-2218.
  • International Conference on Harmonization (2005) Validation of analytical procedures: Text and methodologyICH Q2(R1), International Conference on Harmonization, Geneva, Switzerland.
  • Kaneria, M. and Chanda, S. (2013)’Evaluation of antioxidant and antimicrobial capacity of Syzygium cumini L. leaves extracted sequentially in different solvents’.
  • Food Chemistry, Vol 37, 2, pp. 168-176. https://doi.org/10.1111/j.1745-4514.2011.00614.x
  • Kavitha, K., Murali, M. and Jayachandra, K. (2011) ‘Preliminary Phytochemical Screening, Anthelmintic Activity of Methanolic and Aqueous Extract of Syzygium Cumini Linn.
  • Bark (Myrtaceae)’. Journal of Pharmaceutical Sciences and Research, Vol 3, 9, pp. 1460-1465.
  • Kumar, A., Ilavarasan, R., Jayachandran, T., Deecaraman, M., Kumar, R.M., et al. (2008)’Antiinflammatory activity of Syzygium cumini seed’. African Journal of Biotechnology, Vol 7, 8, pp. 941-943.
  • Murti, K., Paliwal, D., Madan, S., Kundu, R. and Kaushik, M. (2012) ‘Exploration of PreliminaryPhytochemical Studies of Seed of Syzygium cumini’. American Journal of Pharmacology and Toxicology, Vol 7, 1, pp. 12-14. https://doi.org/10.3844/ajptsp.2012.12.14
  • Pareek, A. and Meena, R.K. (2015)’Antimicrobial Activity of Syzygium Cumini’. Indian Journal of Applied Research, 5(8), 751-753.
  • Ravi, K., Rajasekaran, S. and Subramanian, S. (2005)’Antihyperlipidemic effect of Eugenia jambolana seed kernel on streptozotocin-induced diabetes in rats’. Food and Chemical Toxicology, Vol 43, 9, pp. 1433-1439. https://doi.org/10.1016/j.fct.2005.04.004
  • Shamkuwar, P.B., Pawar, D.P. and Chauhan, S.S. (2012)’Antidiarrhoeal activity of seeds of Syzygium cumini L’. Journal of Pharmacy Research, Vol 5, 12, pp. 5537-5539.
  • Shinde, J., Taldone, T., Barletta, M., Kunaparaju, N., Hu, B., et al. (2008)’á-Glucosidase inhibitory activity of Syzygium cumini (Linn.) Skeels seed kernel in vitro and in Goto-Kakizaki (GK) rats’. Carbohydrate Research, Vol 343, pp. 1278-1281. https://doi.org/10.1016/j.carres.2008.03.003